Functional Assay (FA)
(Proliferation of 3T3 cells in response to Mouse PDGF-AA protein)
PDGFAA active protein
PDGFAA protein (Mouse)
Gene Names
pdgfaa; pdgfa; pdgfa-2; zgc:100777
Applications
Functional Assay
Purity
PDGFAA protein was purified by affinity chromatography.
Synonyms
PDGFAA; PDGFAA protein (Mouse); Platelet Derived Growth Factor AA protein; PDGF-1 protein; PDGFAA active protein
Host
Mouse
Specificity
Human
Purity/Purification
PDGFAA protein was purified by affinity chromatography.
Form/Format
Supplied as a sterile liquid with phosphate bufferedsaline, 1 mM DTT, 1% BSA, pH 7.2. 0.22 umfiltered.
Sequence Length
194
Applicable Applications for PDGFAA active protein
Functional Assay
Application Notes
The recombinant mouse IFN-alpha2 has been tested for inhibition of the cytopathic effect of EMC virus on L929 cells. The ED50 for this effect is typically 0.1 ng/ml, corresponding to a specific activity of 1 x 10? U/mg.
Protein Type
Recombinant
Biological Significance
The platelet-derived growth factor AA is a member of the PDGF family. The PDGF proteins are derived from four genes that form four disulfide-linked homodimers and one heterodimer. PDGF-AA plays an important role as a mitogen for a number of cell types. Recombinant Mouse PDGF-AA produced in E Coli is a non-glycosylated homodimer of 126 amino acids with a molecular weight of 28.9 kDa.
Bioactivity
The ED50 of this protein, asmeasured by balb/c-3T3 cell proliferation, is0.1-1.25 ng/ml. This corresponds to a specific activity of 1 x 107 - 8 x 105 Units/mg.
Endotoxin Levels
Less than 0.01 ng/ug cytokine as determined by the LAL assay.
Expression System
E Coli
Preparation and Storage
Store at -70 degree C or less.
Functional Assay (FA)
(Proliferation of 3T3 cells in response to Mouse PDGF-AA protein)
Functional Assay (FA)
(Proliferation of 3T3 cells in response to Mouse PDGF-AA protein)
Related Product Information for PDGFAA active protein
Purified recombinant Mouse PDGFAA protein
Product Categories/Family for PDGFAA active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
platelet-derived growth factor alpha a
NCBI Official Synonym Full Names
platelet-derived growth factor alpha polypeptide a
NCBI Official Symbol
pdgfaa
NCBI Official Synonym Symbols
pdgfa; pdgfa-2; zgc:100777
NCBI Protein Information
platelet-derived growth factor alpha a
Research Articles on PDGFAA
Similar Products
Product Notes
The PDGFAA (Catalog #AAA5304241) is an Active Protein produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PDGFAA can be used in a range of immunoassay formats including, but not limited to, Functional Assay. The recombinant mouse IFN-alpha2 has been tested for inhibition of the cytopathic effect of EMC virus on L929 cells. The ED50 for this effect is typically 0.1 ng/ml, corresponding to a specific activity of 1 x 10? U/mg. Researchers should empirically determine the suitability of the PDGFAA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDGFAA, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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