Rabbit anti-Human, Mouse PRMT5 Polyclonal Antibody | anti-PRMT5 antibody

PRMT5 (Protein arginine N Methyltransferase 5, Shk1 Kinase-binding Protein 1 Homolog, Jak-binding Protein 1, JBP1)

Cat. Num. AAA614939

• Gene Names: PRMT5; JBP1; SKB1; IBP72; SKB1Hs; HRMT1L5
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: PRMT5; Polyclonal Antibody; PRMT5 (Protein arginine N Methyltransferase 5; Shk1 Kinase-binding Protein 1 Homolog; Jak-binding Protein 1; JBP1); Anti -PRMT5 (Protein arginine N Methyltransferase 5; anti-PRMT5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes PRMT5, Mr ~70kD. Species Crossreactivity: Human and mouse.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.

• Applicable Applications for anti-PRMT5 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Western Blot and Immunoprecipitation.; Dilution: Immunoprecipitation: An independent laboratory has reported that this antibody immunoprecipitates active PRMT5 from HeLa cell lysate.
• Immunogen: KLH-conjugated, synthetic peptide corresponding to aa60-79 (KNRPGPQTRSDLLLSGRDWN) of human protein arginine methyltransferase 5, SKB1 homolog. The immunizing sequence is identical in mouse.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot analysis: Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with MBS614939 (0.5ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates PRMT5 (70kD).)

Product Categories/Family for anti-PRMT5 antibody

Antibodies; Abs to Enzymes; Methyltransferase

NCBI and Uniprot Product Information

• NCBI GI #: 88900507
• NCBI GeneID: 10419
• NCBI Accession #: NP_001034708.1
• NCBI GenBank Nucleotide #: NM_001039619.1
• UniProt Accession #: O14744; Q6IBR1; Q9UKH1; B5BU10; D3DS33
• Molecular Weight: 72,684 Da
• NCBI Official Full Name: protein arginine N-methyltransferase 5 isoform b
• NCBI Official Synonym Full Names: protein arginine methyltransferase 5
• NCBI Official Symbol: PRMT5
• NCBI Official Synonym Symbols: JBP1; SKB1; IBP72; SKB1Hs; HRMT1L5
• NCBI Protein Information: protein arginine N-methyltransferase 5; SKB1 homolog; OTTHUMP00000027922; OTTHUMP00000164558; jak-binding protein 1; 72 kDa ICln-binding protein; HMT1 hnRNP methyltransferase-like 5; shk1 kinase-binding protein 1 homolog; histone-arginine N-methyltransferase PRMT5
• UniProt Protein Name: Protein arginine N-methyltransferase 5
• Protein Family: Protein arginine N-methyltransferase
• UniProt Gene Name: PRMT5
• UniProt Synonym Gene Names: HRMT1L5; IBP72; JBP1; SKB1; SKB1 homolog; SKB1Hs
• UniProt Entry Name: ANM5_HUMAN

NCBI Description

This gene encodes an enzyme that belongs to the methyltransferase family. The encoded protein catalyzes the transfer of methyl groups to the amino acid arginine, in target proteins that include histones, transcriptional elongation factors and the tumor suppressor p53. This gene plays a role in several cellular processes, including transcriptional regulation, and the assembly of small nuclear ribonucleoproteins. A pseudogene of this gene has been defined on chromosome 4. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]

Uniprot Description

PRMT5: an ubiquitous enzyme with protein methyltransferase activity. Methylates specific arginine residues in the transcription elongation factor SPT5, and the small nuclear ribonucleoproteins SNRPD1 and SNRPD3. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SPT5 transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Component of the methylosome, a 20S complex that includes ICLN, SKB1 and WDR77. Component of a high molecular weight E2F-pocket protein complex, CERC (cyclin E1 repressor complex). Also interacts with Sm proteins, JAK2, SSTR1 and SPT5. Associates with SWI/SNF remodeling complexes containing SMARCA2 and SMARCA4.

Protein type: Methyltransferase; Methyltransferase, protein arginine; EC 2.1.1.125; RNA processing; Cell cycle regulation

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus

Molecular Function: methyltransferase activity; ribonucleoprotein binding; protein binding; protein-arginine omega-N symmetric methyltransferase activity; histone-arginine N-methyltransferase activity; chromatin binding; transcription corepressor activity

Biological Process: peptidyl-arginine methylation, to symmetrical-dimethyl arginine; establishment and/or maintenance of chromatin architecture; spliceosomal snRNP biogenesis; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; endothelial cell activation; cell proliferation; peptidyl-arginine methylation; regulation of transcription, DNA-dependent; regulation of mitosis; gene expression; circadian regulation of gene expression; peptidyl-arginine N-methylation