Rabbit PRMT3 Polyclonal Antibody | anti-PRMT3 antibody

PRMT3 Antibody (C-term)

Cat. Num. AAA9212943

• Gene Names: PRMT3; HRMT1L3
• Reactivity: Human (Predicted Reactivity: Mouse)
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: PRMT3; Polyclonal Antibody; PRMT3 Antibody (C-term); Protein arginine N-methyltransferase 3; 211-; Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3; HRMT1L3; anti-PRMT3 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Mouse)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This PRMT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 460-491 amino acids from the C-terminal region of human PRMT3.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 2 (varies by lot)
• Sequence Positions: 460-491
• Sequence Length: 531

• Applicable Applications for anti-PRMT3 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of anti-PRMT3 Pab in whole HL60 cell lysate: PRMT3 (Arrow) was detected using purified Pab (Lane B) but not pre-immune serum (lane A). Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PRMT3 Antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Related Product Information for anti-PRMT3 antibody

Arginine methylation is an irreversible post translational modification which has only recently been linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These protein include nuclear proteins, IL enhancer binding factor, nuclear factors, cell cycle proteins, signal transduction proteins, apoptosis proteins, and viral proteins. The mammalian PRMT family currently consists of 7 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all 7 PRMT members have been developed.

NCBI and Uniprot Product Information

• NCBI GI #: 44771198
• NCBI GeneID: 10196
• NCBI Accession #: NP_005779.1
• NCBI GenBank Nucleotide #: NM_005788.3
• UniProt Accession #: O60678; B4DUC7
• Molecular Weight: 59876
• NCBI Official Full Name: protein arginine N-methyltransferase 3 isoform 1
• NCBI Official Synonym Full Names: protein arginine methyltransferase 3
• NCBI Official Symbol: PRMT3
• NCBI Official Synonym Symbols: HRMT1L3
• NCBI Protein Information: protein arginine N-methyltransferase 3
• UniProt Protein Name: Protein arginine N-methyltransferase 3
• Protein Family: Protein arginine N-methyltransferase
• UniProt Gene Name: PRMT3
• UniProt Synonym Gene Names: HRMT1L3
• UniProt Entry Name: ANM3_HUMAN

NCBI Description

This gene belongs to the protein arginine methyltransferase (PRMT) family. The encoded enzyme catalyzes the methylation of guanidino nitrogens of arginyl residues of proteins. The enzyme acts on 40S ribosomal protein S2 (rpS2), which is its major in-vivo substrate, and is involved in the proper maturation of the 80S ribosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

PRMT3: Methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in some proteins. Belongs to the protein arginine N-methyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.1.1.-; Methyltransferase; Methyltransferase, protein arginine; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 11p15.1

Cellular Component: cytoplasm; ribosome; cytosol

Molecular Function: methyltransferase activity; protein-arginine omega-N asymmetric methyltransferase activity; protein-arginine N-methyltransferase activity; protein binding; metal ion binding; histone-arginine N-methyltransferase activity

Biological Process: regulation of transcription, DNA-dependent; negative regulation of protein ubiquitination; peptidyl-arginine methylation, to asymmetrical-dimethyl arginine