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Western Blot (WB) (Used in Western Blot, Sample: Recombinant PRM1, Human)

PRM1 Polyclonal Antibody | anti-PRM1 antibody

Rabbit Anti-PRM1

Applications
ELISA, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Purified by immunoaffinity chromatography.
Synonyms
PRM1; Polyclonal Antibody; Rabbit Anti-PRM1; Protamine 1; CT94.1; Cancer/Testis Antigen Family 94; Member 1; Sperm protamine P1; Cysteine-rich protamine; anti-PRM1 antibody
Ordering
For Research Use Only!
Host
Host: Rabbit; Source: Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human PRM1.
Purity/Purification
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Applicable Applications for anti-PRM1 antibody
ELISA (EIA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Crossreactivity
Human
Immunogen
Recombinant protein corresponding to Ala2-His51of human PRM1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Used in Western Blot, Sample: Recombinant PRM1, Human)

Western Blot (WB) (Used in Western Blot, Sample: Recombinant PRM1, Human)

Immunohistochemistry (IHC)

(Used in DAB staining on formalin fixed paraffin- embedded testis tissue)

Immunohistochemistry (IHC) (Used in DAB staining on formalin fixed paraffin- embedded testis tissue)
Related Product Information for anti-PRM1 antibody
Protamines are small, arginine-rich, nuclear proteins that replace histones late in the haploid phase of spermatogenesis and are believed essential for sperm head condensation and DNA stabilization. Mice, humans, and certain fish have 2 or more different protamines, whereas the sperm of bull, boar, rat, rabbit, guinea pig, and ram have one form of protamine. The 2 human protamines are denoted P1 and P2 (PRM2). PRM1 and PRM2 have a single intron, consisting of 91 and 163 bp, respectively. Both genes were found to contain typical TATAA and CAAT boxes at conventional distances from the transcription start points which, by use of primary extension experiments, were assigned to nucleotides -91 and -110 for PRM1 and PRM2, respectively

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Product Notes

The PRM1 (Catalog #AAA6506756) is an Antibody produced from Host: Rabbit; Source: Human and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PRM1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blot (WB). ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the PRM1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRM1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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