Rabbit anti-Human PRL Polyclonal Antibody | anti-PRL antibody

PRL (S163) (Prolactin) (Azide free) (HRP)

Cat. Num. AAA6494637

• Gene Names: PRL; GHA1
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: PRL; Polyclonal Antibody; PRL (S163) (Prolactin) (Azide free) (HRP); anti-PRL antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PRL.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PRL antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the S163 region of human PRL (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PRL (arrow) using rabbit polyclonal Phospho-PRL-S163.ctrl. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRL gene (Lane 2).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human breast carcinoma with Phospho-PRL-pS163 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of PRL Antibody (S163) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-PRL antibody

Antibodies; Hormones; Steroids

References

1. Liu, M.L., Int J Artif Organs 31(4), 303-308 (2008). 2. Carver, K.C., Mol. Cancer Res. 6(4), 634-643 (2008). 3. Swaminathan, G., J. Endocrinol. 196(2), R1-R7 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4506105
• NCBI GeneID: 5617
• NCBI Accession #: NP_000939
• NCBI GenBank Nucleotide #: NM_000948.5
• UniProt Accession #: P01236; Q15199; Q92996
• Molecular Weight: 23 kDa (200 aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: prolactin
• NCBI Official Synonym Full Names: prolactin
• NCBI Official Symbol: PRL
• NCBI Official Synonym Symbols: GHA1
• NCBI Protein Information: prolactin
• UniProt Protein Name: Prolactin
• UniProt Gene Name: PRL
• UniProt Synonym Gene Names: PRL
• UniProt Entry Name: PRL_HUMAN

NCBI Description

This gene encodes the anterior pituitary hormone prolactin. This secreted hormone is a growth regulator for many tissues, including cells of the immune system. It may also play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2011]

Uniprot Description

prolactin: Prolactin acts primarily on the mammary gland by promoting lactation. Belongs to the somatotropin/prolactin family.

Protein type: Secreted; Cytokine; Secreted, signal peptide; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: extracellular region

Molecular Function: protein binding; prolactin receptor binding; hormone activity

Biological Process: lactation; cell proliferation; cell surface receptor linked signal transduction; regulation of multicellular organism growth; positive regulation of JAK-STAT cascade; female pregnancy