Rabbit PRKG2 Polyclonal Antibody | anti-PRKG2 antibody

PRKG2 Rabbit pAb

Cat. Num. AAA4759550

• Gene Names: PRKG2; cGKII; PRKGR2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: PRKG2; Polyclonal Antibody; PRKG2 Rabbit pAb; protein kinase; cGMP-dependent; type II; cGKII; PRKGR2; anti-PRKG2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: 0.2mg/ml (varies by lot)

• Applicable Applications for anti-PRKG2 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:200-1:1000; IHC-P: 1:25-1:100
• Immunogen: Synthetic peptide of human PRKG2
• Conjugation: Unconjugated
• Modification: Unmodified
• Preparation and Storage: Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western Blot analysis of PRKG2 in Mouse brain and lung tissue in order using PRKG2 Rabbit pAb at dilution 1/160)

Immunohistochemistry (IHC)

(Immunohistochemistry of PRKG2 in paraffin-embedded Human liver cancer tissue using PRKG2 Rabbit pAb at dilution 1/20. Predicted cell location: Cytoplasm)

Related Product Information for anti-PRKG2 antibody

cGKII (cGMP-dependent protein kinase type II) is a major receptor of intracellular cGMP and mediates a plethora of physiological responses. cGKII contains a conserved leucine zipper motif at the amino-terminus. It is expressed in small intestine, colon, prostate, and human brain tissues, and the cGKII gene maps to chromosome 4q13.1-q21.1. cGKII has been shown to regulate the ion transport system in the intestine. Myristoylation of the penultimate glycine in cGKII appears to be essential for directing cGKII to the membrane, since cGKII is devoid of any hydrophobic transmembrane domains. The translocation of cGKII from the cytosol to the membrane allows it to function properly in regulating intestinal ion transport.

NCBI and Uniprot Product Information

• NCBI GI #: 5453978
• NCBI GeneID: 5593
• NCBI Accession #: NP_006250.1
• NCBI GenBank Nucleotide #: NM_006259.1
• UniProt Accession #: Q13237; O00125; O60916
• Molecular Weight: 87,432 Da
• NCBI Official Full Name: cGMP-dependent protein kinase 2
• NCBI Official Synonym Full Names: protein kinase, cGMP-dependent, type II
• NCBI Official Symbol: PRKG2
• NCBI Official Synonym Symbols: cGKII; PRKGR2
• NCBI Protein Information: cGMP-dependent protein kinase 2; cGK2; cGK 2; cGMP-dependent protein kinase II
• UniProt Protein Name: cGMP-dependent protein kinase 2
• Protein Family: cGMP-dependent protein kinase
• UniProt Gene Name: PRKG2
• UniProt Synonym Gene Names: PRKGR2; cGK 2; cGK2; cGKII
• UniProt Entry Name: KGP2_HUMAN

NCBI Description

This gene encodes a protein that belongs to the serine/threonine protein kinase family of proteins. The encoded protein plays a role in the regulation of fluid balance in the intestine. A similar protein in mouse is thought to regulate differentiation and proliferation of cells in the colon. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Uniprot Description

PKG2: an AGC kinase of the PKG family. Type II cGMP-dependent protein kinase is expressed at high levels in brain, lung and intestinal mucosa.

Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.12; Kinase, protein; Protein kinase, AGC; AGC group; PKG family

Chromosomal Location of Human Ortholog: 4q13.1-q21.1

Cellular Component: nuclear membrane; apical plasma membrane; cytosol

Molecular Function: protein homodimerization activity; cGMP-dependent protein kinase activity; cGMP binding; ATP binding; protein kinase activity

Biological Process: circadian regulation of gene expression; regulation of nitric-oxide synthase activity; signal transduction; blood coagulation; protein amino acid phosphorylation; nitric oxide metabolic process