Rabbit RGS20 Polyclonal Antibody | anti-RGS20 antibody

RGS20 Polyclonal Antibody

Cat. Num. AAA3006475

• Gene Names: RGS20; RGSZ1; ZGAP1; gz-GAP; g(z)GAP
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: RGS20; Polyclonal Antibody; RGS20 Polyclonal Antibody; Regulator of G-protein signaling 20; Gz-selective GTPase-activating protein; G(z)GAP; Gz-GAP; Regulator of G-protein signaling Z1; Regulator of Gz-selective protein signaling 1; RGSZ1; ZGAP1; anti-RGS20 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RGS20 polyclonal antibody detects endogenous levels of RGS20 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 388

• Applicable Applications for anti-RGS20 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human RGS20.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of RGS20 polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:NIH-3T3 whole cell lysateLane3:H9C2 whole cell lysate)

Related Product Information for anti-RGS20 antibody

The regulators of G protein signaling (RGS) proteins inhibit heterotrimeric G protein signaling. RGS proteins work by functioning as GTPase-activating proteins (which increase the GTPase activity of G protein a subunits) thereby driving G proteins into their inactive GDP-bound form. RGS20 is expressed exclusively in brain, with highest levels in the caudate nucleus and temporal lobe. RGS20 belongs to the RZ subfamily because it is highly selectivie for the az subunit on G proteins. However, if protein kinase C phosphorylates the az subunit, the G protein is much less susceptible to RGS20 action. RGS20 directly interacts with the microtubule-destabilizing protein SCG10 (superior cervical ganglia, neural specific 10) and blocks its ability to induce microtubule disassmebly.

NCBI and Uniprot Product Information

• NCBI GI #: 41281805
• NCBI GeneID: 8601
• NCBI Accession #: NP_733466.1
• NCBI GenBank Nucleotide #: NP_733466.1
• UniProt Accession #: O76081
• Molecular Weight: ~ 30kDa
• NCBI Official Full Name: regulator of G-protein signaling 20 isoform a
• NCBI Official Synonym Full Names: regulator of G protein signaling 20
• NCBI Official Symbol: RGS20
• NCBI Official Synonym Symbols: RGSZ1; ZGAP1; gz-GAP; g(z)GAP
• NCBI Protein Information: regulator of G-protein signaling 20
• UniProt Protein Name: Regulator of G-protein signaling 20
• Protein Family: Regulator of G-protein signaling
• UniProt Gene Name: RGS20
• UniProt Synonym Gene Names: RGSZ1; ZGAP1
• UniProt Entry Name: RGS20_HUMAN

NCBI Description

The protein encoded by this gene belongs to the family of regulator of G protein signaling (RGS) proteins, which are regulatory and structural components of G protein-coupled receptor complexes. RGS proteins inhibit signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound forms. This protein selectively binds to G(z)-alpha and G(alpha)-i2 subunits, and regulates their signaling activities. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

RGS20: Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds selectively to G(z)-alpha and G(alpha)-i2 subunits, accelerates their GTPase activity and regulates their signaling activities. The G(z)-alpha activity is inhibited by the phosphorylation and palmitoylation of the G- protein. Negatively regulates mu-opioid receptor-mediated activation of the G-proteins. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: GAPs, misc.; GAPs

Chromosomal Location of Human Ortholog: 8q11.23

Cellular Component: cytoplasm; plasma membrane; nucleus

Molecular Function: protein binding; GTPase activator activity

Biological Process: regulation of G-protein coupled receptor protein signaling pathway; positive regulation of GTPase activity