Rabbit anti-Human, Rat PRKAR1A Polyclonal Antibody | anti-PRKAR1A antibody

Anti-PRKAR1A Antibody

Cat. Num. AAA178619

• Gene Names: PRKAR1A; CAR; CNC; CNC1; PKR1; TSE1; ADOHR; PPNAD1; PRKAR1; ACRDYS1
• Reactivity: Human, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified.
• Synonyms: PRKAR1A; Polyclonal Antibody; Anti-PRKAR1A Antibody; ACRDYS1; ADOHR; CAR; CNC 1; CNC; CNC1; PKA RIA; PKR 1; PKR1; PPNAD 1; PPNAD1; PRKAR 1; PRKAR1; TSE 1; TSE1; P10644; cAMP-dependent protein kinase type I-alpha regulatory subunit; protein kinase cAMP-dependent type I regulatory subunit alpha; anti-PRKAR1A antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 381

• Applicable Applications for anti-PRKAR1A antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot: 0.1-0.5ug/ml; Immunohistochemistry(IHC) Paraffin: 0.5-1ug/ml
• Notes: Tested Species: In-house tested species with positive results.; By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.; Other applications have not been tested.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Immunogen: E Coli-derived human PRKAR1A recombinant protein (Position: E2-E81). Human PRKAR1A shares 89.9% and 92.4% amino acid (aa) sequence identity with mouse and rat PRKAR1A, respectively.
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Western blot analysis of PRKAR1A expression in rat thymus extract (lane 1), HEPG-2 whole cell lysates (lane 2) and MCF-7 whole cell lysates (lane 3). PRKAR1A at 48KD was detected using rabbit anti- PRKAR1A Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method. )

Immunohistochemistry (IHC)

(PRKAR1A was detected in paraffin-embedded sections of rat testis tissues using rabbit anti- PRKAR1A Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method. )

Immunohistochemistry (IHC)

(PRKAR1A was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti- PRKAR1A Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method. )

Immunohistochemistry (IHC)

(PRKAR1A was detected in paraffin-embedded sections of human lung cancer tissues using rabbit anti- PRKAR1A Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method. )

Related Product Information for anti-PRKAR1A antibody

Rabbit IgG polyclonal antibody for cAMP-dependent protein kinase type I-alpha regulatory subunit(PRKAR1A) detection.
Background: cAMP-dependent protein kinase type I-alpha regulatory subunit is an enzyme that in humans is encoded by the PRKAR1A gene. This protein encoded by this gene was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Mutations in this gene cause Carney complex (CNC). This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. A nonconventional nuclear localization sequence (NLS) has been found for this protein which suggests a role in DNA replication via the protein serving as a nuclear transport protein for the second subunit of the Replication Factor C (RFC40). Several alternatively spliced transcript variants encoding two different isoforms have been observed.

References

1. "Entrez Gene: PRKAR1A protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1)".
2. Scambler P, Oyen O, Wainwright B, Farrall M, Law HY, Estivill X, Sandberg M, Williamson R, Jahnsen T (December 1987). "Exclusion of catalytic and regulatory subunits of cAMP-dependent protein kinase as candidate genes for the defect causing cystic fibrosis". Am J Hum Genet. 41 (5): 925-32.

NCBI and Uniprot Product Information

• NCBI GI #: 443497964
• NCBI GeneID: 5573
• NCBI Accession #: NP_001263218.1
• NCBI GenBank Nucleotide #: NM_001276289.1
• UniProt Accession #: P10644; Q567S7; K7ER48
• Molecular Weight: 38,003 Da
• NCBI Official Full Name: cAMP-dependent protein kinase type I-alpha regulatory subunit isoform a
• NCBI Official Synonym Full Names: protein kinase cAMP-dependent type I regulatory subunit alpha
• NCBI Official Symbol: PRKAR1A
• NCBI Official Synonym Symbols: CAR; CNC; CNC1; PKR1; TSE1; ADOHR; PPNAD1; PRKAR1; ACRDYS1
• NCBI Protein Information: cAMP-dependent protein kinase type I-alpha regulatory subunit
• UniProt Protein Name: cAMP-dependent protein kinase type I-alpha regulatory subunit
• Protein Family: cAMP-dependent protein kinase
• UniProt Gene Name: PRKAR1A
• UniProt Synonym Gene Names: PKR1; PRKAR1; TSE1; TSE1

NCBI Description

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. This gene encodes one of the regulatory subunits. This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Mutations in this gene cause Carney complex (CNC). This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. A nonconventional nuclear localization sequence (NLS) has been found for this protein which suggests a role in DNA replication via the protein serving as a nuclear transport protein for the second subunit of the Replication Factor C (RFC40). Several alternatively spliced transcript variants encoding two different isoforms have been observed. [provided by RefSeq, Jan 2013]

Uniprot Description

PKAR1A: a regulatory subunit of cAMP-regulated protein kinase. The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible. Interacts with RFC2: the complex may be involved in cell survival. Defects in PRKAR1A are the cause of Carney complex type 1 (CNC1). CNC is a multiple neoplasia syndrome characterized by spotty skin pigmentation, cardiac and other myxomas, endocrine tumors, and psammomatous melanotic schwannomas.

Protein type: Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 17q24.2

Cellular Component: AMP-activated protein kinase complex; cytoplasm; cytosol; immunological synapse; membrane; protein complex

Molecular Function: cAMP-dependent protein kinase inhibitor activity; cAMP-dependent protein kinase regulator activity; protein binding; ubiquitin protein ligase binding

Biological Process: activation of protein kinase A; blood coagulation; negative regulation of activated T cell proliferation; regulation of transcription from RNA polymerase II promoter; renal water homeostasis

Disease: Acrodysostosis 1 With Or Without Hormone Resistance; Carney Complex, Type 1; Myxoma, Intracardiac; Pigmented Nodular Adrenocortical Disease, Primary, 1; Thyroid Carcinoma, Papillary