Rabbit anti-Human PRKAG3 Polyclonal Antibody | anti-PRKAG3 antibody

PRKAG3 Antibody

Cat. Num. AAA1492697

• Gene Names: PRKAG3; AMPKG3
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Antigen Affinity Purified
• Synonyms: PRKAG3; Polyclonal Antibody; PRKAG3 Antibody; AMPK gamma3; AMPK subunit gamma-3; AMPKG3; anti-PRKAG3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Sequence Length: 489

• Applicable Applications for anti-PRKAG3 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Recommended dilution: WB:1:500-2000
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Conjugate: Non-conjugated
• Immunogen: Recombinant human 5'-AMP-activated protein kinase subunit gamma-3 protein (1-210aa)
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-19145 / sc-19146 / sc-20166 / sc-130687
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(All lanes: 5-AMP-activated protein kinase subunit gamma-3 antibody at 5ug/mlLane 1:Hela whole cell lysateLane 2:HepG2 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 55,52 kDaObserved band size: 54 kDa)

Related Product Information for anti-PRKAG3 antibody

AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.

NCBI and Uniprot Product Information

• NCBI GI #: 47132577
• NCBI GeneID: 53632
• NCBI Accession #: NP_059127.2
• NCBI GenBank Nucleotide #: NM_017431.2
• UniProt Accession #: Q9UGI9; Q4QQG8; Q4V779; Q9NRL1
• Molecular Weight: 51,484 Da
• NCBI Official Full Name: 5'-AMP-activated protein kinase subunit gamma-3
• NCBI Official Synonym Full Names: protein kinase AMP-activated non-catalytic subunit gamma 3
• NCBI Official Symbol: PRKAG3
• NCBI Official Synonym Symbols: AMPKG3
• NCBI Protein Information: 5'-AMP-activated protein kinase subunit gamma-3
• UniProt Protein Name: 5'-AMP-activated protein kinase subunit gamma-3
• Protein Family: 5'-AMP-activated protein kinase
• UniProt Gene Name: PRKAG3
• UniProt Synonym Gene Names: AMPKG3; AMPK gamma3; AMPK subunit gamma-3
• UniProt Entry Name: AAKG3_HUMAN

NCBI Description

The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. It is dominantly expressed in skeletal muscle. Studies of the pig counterpart suggest that this subunit may play a key role in the regulation of energy metabolism in skeletal muscle. [provided by RefSeq, Jul 2008]

Uniprot Description

AMPKG3: AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive. AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non- catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2. Skeletal muscle, with weak expression in heart and pancreas. Belongs to the 5'-AMP-activated protein kinase gamma subunit family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: AMP-activated protein kinase complex; cytosol; extracellular space

Molecular Function: AMP-activated protein kinase activity; ATP binding; protein kinase binding

Biological Process: cell cycle arrest; fatty acid biosynthetic process; gene expression; glucose transport; glycogen biosynthetic process; insulin receptor signaling pathway; macroautophagy; mitochondrion organization and biogenesis; organelle organization and biogenesis; protein amino acid phosphorylation; transcription initiation from RNA polymerase II promoter