Rabbit anti-Human PRKACA Polyclonal Antibody | anti-PRKACA antibody

PRKACA, NT (K82) (cAMP-dependent Protein Kinase Catalytic Subunit alpha, PKA C-alpha, PKACA) (Biotin)

Cat. Num. AAA6495094

• Gene Names: PRKACA; PKACA; PPNAD4
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: PRKACA; Polyclonal Antibody; NT (K82) (cAMP-dependent Protein Kinase Catalytic Subunit alpha; PKA C-alpha; PKACA) (Biotin); anti-PRKACA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PRKACA.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-PRKACA antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal(K282) region of human PRKACA (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PRKACA Antibody (N-term K82) in NCI-H460 cell line lysates (35ug/lane). PRKACA (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human testis tissue reacted with PRKACA Antibody (N-term K82), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(PRKACA Antibody (N-term K82) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-PRKACA antibody

Antibodies; Protein Kinases

References

1. Sugiyama, H., et.al., J. Biol. Chem. 267(35), 25256-25263 (1992).

NCBI and Uniprot Product Information

• NCBI GI #: 4506055
• NCBI GeneID: 5566
• NCBI Accession #: NP_002721
• NCBI GenBank Nucleotide #: NM_002730.3
• UniProt Accession #: P17612; Q32P54; Q9H2Y0; Q9NRB4; Q9NRH9
• Molecular Weight: 67.0kDa (578aa) 50-70KDa (SDS-PAGE under reducing conditions.)
• NCBI Official Full Name: cAMP-dependent protein kinase catalytic subunit alpha isoform Calpha1
• NCBI Official Synonym Full Names: protein kinase cAMP-activated catalytic subunit alpha
• NCBI Official Symbol: PRKACA
• NCBI Official Synonym Symbols: PKACA; PPNAD4
• NCBI Protein Information: cAMP-dependent protein kinase catalytic subunit alpha
• UniProt Protein Name: cAMP-dependent protein kinase catalytic subunit alpha
• Protein Family: cAMP-dependent protein kinase
• UniProt Gene Name: PRKACA
• UniProt Synonym Gene Names: PKACA; PKA C-alpha
• UniProt Entry Name: KAPCA_HUMAN

NCBI Description

This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms. [provided by RefSeq, Jan 2015]