Western Blot (WB)
(Western blot analysis of PRKACA Antibody (N-term K82) in NCI-H460 cell line lysates (35ug/lane). PRKACA (arrow) was detected using the purified Pab.)
Rabbit anti-Human PRKACA Polyclonal Antibody | anti-PRKACA antibody
PRKACA, NT (K82) (cAMP-dependent Protein Kinase Catalytic Subunit alpha, PKA C-alpha, PKACA)
Reactivity
Human
Applications
ELISA, Western Blot
Purity
PurifiedPurified by ammonium sulfate precipitation.
Synonyms
PRKACA; Polyclonal Antibody; NT (K82) (cAMP-dependent Protein Kinase Catalytic Subunit alpha; PKA C-alpha; PKACA); Anti -PRKACA; anti-PRKACA antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human PRKACA.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Purified by ammonium sulfate precipitation.
Form/Format
Supplied as a liquid in PBS, 0.84% sodium azide.
Applicable Applications for anti-PRKACA antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunogen
Synthetic peptide selected from the N-terminal(K282) region of human PRKACA (KLH).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of PRKACA Antibody (N-term K82) in NCI-H460 cell line lysates (35ug/lane). PRKACA (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of PRKACA Antibody (N-term K82) in NCI-H460 cell line lysates (35ug/lane). PRKACA (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human testis tissue reacted with PRKACA Antibody (N-term K82), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human testis tissue reacted with PRKACA Antibody (N-term K82), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(PRKACA Antibody (N-term K82) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(PRKACA Antibody (N-term K82) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-PRKACA antibody
PRKACA phosphorylates a large number of substrates in the cytoplasm and the nucleus.
Product Categories/Family for anti-PRKACA antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
cAMP-dependent protein kinase catalytic subunit alpha
NCBI Official Symbol
PRKACA
NCBI Protein Information
protein kinase, cAMP-dependent, catalytic, alpha
Protein Family
Similar Products
Product Notes
The PRKACA (Catalog #AAA646795) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PRKACA, NT (K82) (cAMP-dependent Protein Kinase Catalytic Subunit alpha, PKA C-alpha, PKACA) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PRKACA can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 1:1,000 Western Blot: 1:50-1:100. Researchers should empirically determine the suitability of the PRKACA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRKACA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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