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Immunohistochemistry (IHC)
(Anti-PRICKLE4 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Rabbit anti-Human PRICKLE4 Polyclonal Antibody | anti-PRICKLE4 antibody
Anti-PRICKLE4 Antibody (N-Terminus) IHC-plus
Gene Names
PRICKLE4; OBTP; OEBT; TOMM6; C6orf49
Reactivity
Human
Applications
Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Western Blot, ELISA
Purity
Immunoaffinity purified
Synonyms
PRICKLE4; Polyclonal Antibody; Anti-PRICKLE4 Antibody (N-Terminus) IHC-plus; Rabbit Polyclonal to Human PRICKLE4; Human PRICKLE4; C6orf49; Prickle homolog 4 (Drosophila); Prickle-like protein 4; OBTP; OEBT; anti-PRICKLE4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Human PRICKLE4. Two isoforms of PRICKLE4 are known to exist. PRICKLE4 antibody is predicted to not cross-react with other PRICKLE protein family members.
Purity/Purification
Immunoaffinity purified
Form/Format
PBS, 0.02% sodium azide
Concentration
1 mg/ml (varies by lot)
Sequence Length
384
Applicable Applications for anti-PRICKLE4 antibody
Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
Application Notes
ICC (5 ug/ml)
IF (5 ug/ml)
IHC-P (5 ug/ml)
WB (0.25 ug/ml)
IF (5 ug/ml)
IHC-P (5 ug/ml)
WB (0.25 ug/ml)
Immunogen
PRICKLE4 antibody was raised against an 18 amino acid peptide near the amino terminus of human PRICKLE4
Immunogen Description
An 18 amino acid peptide near the amino terminus of human PRICKLE4
Immunogen Type
Synthetic peptide
Antigen Modification
N-Terminus
Target Species
Human
Preparation and Storage
Short term 4 degree C
Long term aliquot and store at -20 degree C
Avoid freeze thaw cycles. Store undiluted.
Long term aliquot and store at -20 degree C
Avoid freeze thaw cycles. Store undiluted.
Immunohistochemistry (IHC)
(Anti-PRICKLE4 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Immunohistochemistry (IHC)
(Anti-PRICKLE4 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Immunocytochemistry (ICC)
(Immunocytochemistry of PRICKLE4 in A549 cells with PRICKLE4 antibody at 5 µg/mL.)
Immunocytochemistry (ICC)
(Immunocytochemistry of PRICKLE4 in A549 cells with PRICKLE4 antibody at 5 µg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of PRICKLE4 in A549 cells with PRICKLE4 antibody at 20 µg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of PRICKLE4 in A549 cells with PRICKLE4 antibody at 20 µg/mL.)
Western Blot (WB)
(Western blot analysis of PRICKLE4 in A549 cell lysate with PRICKLE4 antibody at 0.25 ug/ml in the (A) absence and (B) presence of blocking peptide.)
Western Blot (WB)
(Western blot analysis of PRICKLE4 in A549 cell lysate with PRICKLE4 antibody at 0.25 ug/ml in the (A) absence and (B) presence of blocking peptide.)
Related Product Information for anti-PRICKLE4 antibody
PRICKLE4 Antibody, C6orf49 Antibody, Prickle homolog 4 (Drosophila) Antibody, Prickle-like protein 4 Antibody, OBTP Antibody, OEBT Antibody Description: C6ORF49 is a member of the LIM domain protein family (Teufel et al., 2005 [PubMed 15702247]).[supplied by OMIM, Mar 2008]
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
41,766 Da
NCBI Official Full Name
prickle-like protein 4
NCBI Official Synonym Full Names
prickle planar cell polarity protein 4
NCBI Official Symbol
PRICKLE4
NCBI Official Synonym Symbols
OBTP; OEBT; TOMM6; C6orf49
NCBI Protein Information
prickle-like protein 4
UniProt Protein Name
Prickle-like protein 4
Protein Family
UniProt Gene Name
PRICKLE4
UniProt Synonym Gene Names
C6orf49; OEBT
UniProt Entry Name
PRIC4_HUMAN
NCBI Description
C6ORF49 is a member of the LIM domain protein family (Teufel et al., 2005 [PubMed 15702247]).[supplied by OMIM, Mar 2008]
Uniprot Description
PRICKLE4: Belongs to the prickle / espinas / testin family. 3 isoforms of the human protein are produced by alternative splicing
Chromosomal Location of Human Ortholog: 6p21.31
Cellular Component: nucleus
Molecular Function: zinc ion binding
Research Articles on PRICKLE4
Similar Products
Product Notes
The PRICKLE4 prickle4 (Catalog #AAA2400177) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PRICKLE4 Antibody (N-Terminus) IHC-plus reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PRICKLE4 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA). ICC (5 ug/ml) IF (5 ug/ml) IHC-P (5 ug/ml) WB (0.25 ug/ml). Researchers should empirically determine the suitability of the PRICKLE4 prickle4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRICKLE4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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