Immunocytochemistry (ICC)
(Immunocytochemistry: Confocal IF analysis of N1E-115 using MBS620132 (1:500) (Red). Actin filaments have been labeled with Alexa Fluor 488-Phalloidin (Green). Nuclear is stained with DAPI (Blue).)
Rabbit anti-Mouse PRG4 Polyclonal Antibody | anti-PRG4 antibody
PRG4 (Proteoglycan 4) (MaxLight 490)
Gene Names
LPPR2; PRG4
Reactivity
Mouse
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
Purified by Immunoaffinity chromatography.
Synonyms
PRG4; Polyclonal Antibody; PRG4 (Proteoglycan 4) (MaxLight 490); anti-PRG4 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes PRG4, ~37kD. Species Crossreactivity: mouse
Purity/Purification
Purified by Immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.
Applicable Applications for anti-PRG4 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide corresponding to human PRG4 from C-terminal, conjugated to KLH. Species sequence homology: rat, human, primate and equine
Conjugate
MaxLight490
Positive Control
Mouse Brain lysates
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunocytochemistry (ICC)
(Immunocytochemistry: Confocal IF analysis of N1E-115 using MBS620132 (1:500) (Red). Actin filaments have been labeled with Alexa Fluor 488-Phalloidin (Green). Nuclear is stained with DAPI (Blue).)
Immunocytochemistry (ICC)
(Immunocytochemistry: Confocal IF analysis of N1E-115 using MBS620132 (1:500) (Red). Actin filaments have been labeled with Alexa Fluor 488-Phalloidin (Green). Nuclear is stained with DAPI (Blue).)
Testing Data
(Mouse brain (e13-14) tissue lysate was resolved by electrophoresis, transferred to PVDF and probed with MBS620132 (1:2000). Proteins were visualized using an IgG (HRP) donkey anti-rabbit and a chemiluminescence detection system. Arrow indicates PRG4 (37kD))
Testing Data
(Mouse brain (e13-14) tissue lysate was resolved by electrophoresis, transferred to PVDF and probed with MBS620132 (1:2000). Proteins were visualized using an IgG (HRP) donkey anti-rabbit and a chemiluminescence detection system. Arrow indicates PRG4 (37kD))
Related Product Information for anti-PRG4 antibody
PRG4, Proteoglycan 4, is a human gene and is found in synovial tissue, cartilage, and liver. Small amounts are found in the lung. Isoform 1 is found in the kidney, lung, liver, heart and brain. The other Isoforms, C & D, are widely expressed. PRG4 is a lubricating glycoprotein containing chondroitin sulfate and keratan sulfate glycosaminoglycans. It is responsible for boundary lubrication at the cartilage surface. A disease associated with PRG4 is Jacobs's syndrome which is an autosomal recessive disorder.
Product Categories/Family for anti-PRG4 antibody
References
1. Sah, L, et al., (2006). Tissue Engineering. 12(6): 1429-1439.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36,880 Da
NCBI Official Full Name
lipid phosphate phosphatase-related protein type 2 isoform 2
NCBI Official Symbol
LPPR2
NCBI Official Synonym Symbols
PRG4
NCBI Protein Information
lipid phosphate phosphatase-related protein type 2; PRG-4; plasticity related gene 4; plasticity-related gene 4 protein
UniProt Protein Name
Lipid phosphate phosphatase-related protein type 2
Protein Family
UniProt Gene Name
LPPR2
UniProt Synonym Gene Names
PRG4; PRG-4
UniProt Entry Name
LPPR2_HUMAN
Uniprot Description
Catalytic activity: A 1,2-diacylglycerol 3-phosphate + H2O = a 1,2-diacyl-sn-glycerol + phosphate.
Subcellular location: Membrane; Multi-pass membrane protein
Potential.
Sequence similarities: Belongs to the PA-phosphatase related phosphoesterase family.
Similar Products
Product Notes
The PRG4 lppr2 (Catalog #AAA6494276) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PRG4 (Proteoglycan 4) (MaxLight 490) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PRG4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PRG4 lppr2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRG4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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