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Immunohistochemistry (IHC) (P-peptide-+ Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using ACC1 (Phospho-Ser80) antibody (#A0405).)

ACC1 Antibody | anti-ACC1 antibody

ACC1 (Phospho-Ser80) Antibody

Gene Names
ACACA; ACC; ACAC; ACC1; ACCA; ACACAD
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
ACC1; ACC1 (Phospho-Ser80) Antibody; ACAC; ACACA; ACC-alpha; ACCA; Acetyl-CoA carboxylase 1; EC 6.3.4.14; EC 6.4.1.2; anti-ACC1 antibody
Ordering
For Research Use Only!
Reactivity
Human, Mouse, Rat
Specificity
ACC1 (Phospho-Ser80) antibody detects endogenous levels of ACC1 only when phosphorylated at serine 79.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
2383
Applicable Applications for anti-ACC1 antibody
Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
IHC: 1:50~1:100
ELISA: 1:1000
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human ACC1 around the phosphorylation site of serine 79 (S-M-SP-G-L).
All Sites
Human: Ser80
Mouse: Ser79
Rat: Ser79
Preparation and Storage
Store at-20 degree C for 1 year

Immunohistochemistry (IHC)

(P-peptide-+ Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using ACC1 (Phospho-Ser80) antibody (#A0405).)

Immunohistochemistry (IHC) (P-peptide-+ Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using ACC1 (Phospho-Ser80) antibody (#A0405).)

Western Blot (WB)

(Western blot analysis of extracts from K562 cells treated with Insulin (0.01U/ml, 15mins), using ACC1 (Phospho-Ser80) antibody (#A0405).)

Western Blot (WB) (Western blot analysis of extracts from K562 cells treated with Insulin (0.01U/ml, 15mins), using ACC1 (Phospho-Ser80) antibody (#A0405).)
References
Naomoto Harada, Mol. Cell. Biol., Mar 2007; 27: 1881-1888. Naomoto Harada, Mol. Cell. Biol., May 2007; 27: 3556. D. A. Somers, Plant Physiology, Mar 1993; 101: 1097. M. Joachimiak, PNAS, Sep 1997; 94: 9990.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
31
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
269,999 Da
NCBI Official Full Name
acetyl-CoA carboxylase 1 isoform 1
NCBI Official Synonym Full Names
acetyl-CoA carboxylase alpha
NCBI Official Symbol
ACACA
NCBI Official Synonym Symbols
ACC; ACAC; ACC1; ACCA; ACACAD
NCBI Protein Information
acetyl-CoA carboxylase 1
UniProt Protein Name
Acetyl-CoA carboxylase 1
Protein Family
UniProt Gene Name
ACACA
UniProt Synonym Gene Names
ACAC; ACC1; ACCA; ACC1

NCBI Description

Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.

Research Articles on ACC1

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Product Notes

The ACC1 acaca (Catalog #AAA856501) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The ACC1 (Phospho-Ser80) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ACC1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA). IHC: 1:50~1:100 ELISA: 1:1000. Researchers should empirically determine the suitability of the ACC1 acaca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACC1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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