GA Binding Protein Transcription Factor Alpha Recombinant Protein | GABPa recombinant protein

Recombinant GA Binding Protein Transcription Factor Alpha (GABPa)

Cat. Num. AAA2029784

• Gene Names: GABPA; NFT2; NRF2; NRF2A; E4TF1A; E4TF1-60; RCH04A07
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: GA Binding Protein Transcription Factor Alpha; Recombinant GA Binding Protein Transcription Factor Alpha (GABPa); GABPa recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; AAL EGYRKEQERL GIPYDPIQWS TDQVLHWVVWVMKEFSMTDI DLTTLNISGR ELCSLNQEDF FQRVPRGEIL WSHLELLRKYVLASQEQQMN EIVTIDQPVQ IIPASVQSAT PTTIKVINSS AKAAKVQRAPRISGEDRSSP GNRT
• Sequence Length: 454

• Applicable Applications for GABPa recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 20.4kDa
• Accurate Molecular Mass (KD): 20kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and S-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Ala168~Thr314
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 383615289
• NCBI GeneID: 2551
• Molecular Weight: 51,295 Da
• NCBI Official Full Name: GA binding protein transcription factor alpha subunit 60kDa
• NCBI Official Synonym Full Names: GA binding protein transcription factor, alpha subunit 60kDa
• NCBI Official Symbol: GABPA
• NCBI Official Synonym Symbols: NFT2; NRF2; NRF2A; E4TF1A; E4TF1-60; RCH04A07
• NCBI Protein Information: GA-binding protein alpha chain
• UniProt Protein Name: GA-binding protein alpha chain
• Protein Family: GA-binding protein
• UniProt Gene Name: GABPA
• UniProt Synonym Gene Names: E4TF1A; GABP subunit alpha
• UniProt Entry Name: GABPA_HUMAN

NCBI Description

This gene encodes one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit. Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Oct 2010]

Uniprot Description

GABPA: Transcription factor capable of interacting with purine rich repeats (GA repeats). Necessary for the expression of the Adenovirus E4 gene. Belongs to the ETS family.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 21q21.3

Cellular Component: nucleoplasm; nuclear chromatin; nucleus

Molecular Function: protein binding; DNA binding; protein heterodimerization activity; transcription coactivator activity; chromatin binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; mitochondrion organization and biogenesis; in utero embryonic development; organelle organization and biogenesis; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; cell differentiation; negative regulation of megakaryocyte differentiation