Western Blot (WB)
(Western BlotPositive WB detected in: A549 whole cell lysate, Hela whole cell lysate, SH-SY5Y whole cell lysateAll lanes: PRDX6 antibody at 4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 26 KDaObserved band size: 26 KDa)
Rabbit anti-Human PRDX6 Polyclonal Antibody | anti-PRDX6 antibody
PRDX6 Antibody
IHC: 1:20-1:200
IF: 1:50-1:200
IP: 1:200-1:2000
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Western Blot (WB)
(Western BlotPositive WB detected in: A549 whole cell lysate, Hela whole cell lysate, SH-SY5Y whole cell lysateAll lanes: PRDX6 antibody at 4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 26 KDaObserved band size: 26 KDa)
Western Blot (WB)
(Western BlotPositive WB detected in: A549 whole cell lysate, Hela whole cell lysate, SH-SY5Y whole cell lysateAll lanes: PRDX6 antibody at 4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 26 KDaObserved band size: 26 KDa)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human ovarian cancer using MBS7103463 at dilution of 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human ovarian cancer using MBS7103463 at dilution of 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human glioma cancer using MBS7103463 at dilution of 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human glioma cancer using MBS7103463 at dilution of 1:100)
Immunofluorescence (IF)
(Immunofluorescent analysis of Hela cells using MBS7103463 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Immunofluorescence (IF)
(Immunofluorescent analysis of Hela cells using MBS7103463 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Immunoprecipitation (IP)
(Immunoprecipitating PRDX6 in Hela whole cell lysateLane 1: Rabbit control IgG (1ug)instead of MBS7103463 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: MBS7103463 (8ug)+ Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (10ug))
Immunoprecipitation (IP)
(Immunoprecipitating PRDX6 in Hela whole cell lysateLane 1: Rabbit control IgG (1ug)instead of MBS7103463 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: MBS7103463 (8ug)+ Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (10ug))
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. [provided by RefSeq, Jul 2008]
Uniprot Description
Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Can reduce H2O2 and short chain organic, fatty acid, and phospholipid hydroperoxides. Also has phospholipase activity, and can therefore either reduce the oxidized sn-2 fatty acyl grup of phospholipids (peroxidase activity) or hydrolyze the sn-2 ester bond of phospholipids (phospholipase activity). These activities are dependent on binding to phospholipids at acidic pH and to oxidized phospholipds at cytosolic pH. Plays a role in cell protection against oxidative stress by detoxifying peroxides and in phospholipid homeostasis.
Research Articles on PRDX6
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Product Notes
The PRDX6 prdx6 (Catalog #AAA7103463) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PRDX6 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PRDX6 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500-1:5000 IHC: 1:20-1:200 IF: 1:50-1:200 IP: 1:200-1:2000. Researchers should empirically determine the suitability of the PRDX6 prdx6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRDX6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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