Western Blot (WB)
(PRAMEF8 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PRAMEF8 antibody detected the PRAMEF8 protein (arrow).)
Rabbit anti-Human PRAMEF8 Polyclonal Antibody | anti-PRAMEF8 antibody
PRAMEF8 Antibody (C-term)
Gene Names
PRAMEF8; PRAMEF24
Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PRAMEF8; Polyclonal Antibody; PRAMEF8 Antibody (C-term); PRAME family member 8; anti-PRAMEF8 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PRAMEF8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 419-447 amino acids from the C-terminal region of human PRAMEF8.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
419-447
Sequence Length
474
Applicable Applications for anti-PRAMEF8 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(PRAMEF8 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PRAMEF8 antibody detected the PRAMEF8 protein (arrow).)
Western Blot (WB)
(PRAMEF8 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PRAMEF8 antibody detected the PRAMEF8 protein (arrow).)
Immunohistochemistry (IHC)
(PRAMEF8 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human melanoma tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PRAMEF8 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(PRAMEF8 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human melanoma tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PRAMEF8 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(PRAMEF8 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(PRAMEF8 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-PRAMEF8 antibody
References
Gregory, S.G., et al. Nature 441(7091):315-321(2006)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53669
NCBI Official Full Name
PRAME family member 8
NCBI Official Synonym Full Names
PRAME family member 8
NCBI Official Symbol
PRAMEF8
NCBI Official Synonym Symbols
PRAMEF24
NCBI Protein Information
PRAME family member 8
UniProt Protein Name
PRAME family member 8
Protein Family
UniProt Gene Name
PRAMEF8
UniProt Synonym Gene Names
PRAMEF24
UniProt Entry Name
PRAM8_HUMAN
Uniprot Description
PRAMEF8: Belongs to the PRAME family.
Chromosomal Location of Human Ortholog: 1p36.21
Molecular Function: retinoic acid receptor binding
Biological Process: negative regulation of retinoic acid receptor signaling pathway; negative regulation of cell differentiation; positive regulation of cell proliferation; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis
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Product Notes
The PRAMEF8 pramef8 (Catalog #AAA9208686) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 419-447. The PRAMEF8 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PRAMEF8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the PRAMEF8 pramef8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRAMEF8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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