Rabbit PRAF2 Polyclonal Antibody | anti-PRAF2 antibody

Anti-PRAF2 Antibody (aa141-190) IHC-plus

Cat. Num. AAA2400065

• Gene Names: PRAF2; JM4
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot
• Purity: Immunoaffinity purified
• Synonyms: PRAF2; Polyclonal Antibody; Anti-PRAF2 Antibody (aa141-190) IHC-plus; Rabbit Polyclonal to Human PRAF2; Human PRAF2; JM4; Jena-Muenchen 4; PRA1 domain family; member 2; PRA1 family protein 2; PRA1 domain family 2; anti-PRAF2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Human JM4 / PRAF2
• Purity/Purification: Immunoaffinity purified
• Form/Format: PBS, pH 7.2, 0.05% Sodium Azide

• Applicable Applications for anti-PRAF2 antibody: Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB)
• Application Notes: IHC-P: 1:200; IF: 1:50-1:200; WB: 1:500-1:1000; ; Optimal dilution to be determined by the researcher.
• Immunogen: Synthetic peptide around Leu166 of human JM4 / PRAF2.
• Immunogen Species: PRAF2 antibody was raised against Human
• Epitope: aa141-190
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-PRAF2 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-PRAF2 antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Western blot analysis of JM4/PRAF2 pAb at 1:500 dilution. Lane 1: HEK293T cell lysate. Lane 2: Rat brain tissue lysate. Lane 3: Mouse brain tissue lysate.)

Western Blot (WB)

(Western blot of JM4/PRAF2 (L166) pAb in extracts from LOVO cells.)

Related Product Information for anti-PRAF2 antibody

PRAF2 Antibody, JM4 Antibody, Jena-Muenchen 4 Antibody, PRA1 domain family, member 2 Antibody, PRA1 family protein 2 Antibody, PRA1 domain family 2 Antibody Description: May be involved in ER/Golgi transport and vesicular traffic. Plays a proapoptic role in cerulenin-induced neuroblastoma apoptosis.

NCBI and Uniprot Product Information

• NCBI GI #: 6005794
• NCBI GeneID: 11230
• NCBI Accession #: NP_009144.1
• NCBI GenBank Nucleotide #: NM_007213.2
• UniProt Accession #: O60831; B2RD20
• NCBI Official Full Name: PRA1 family protein 2
• NCBI Official Synonym Full Names: PRA1 domain family member 2
• NCBI Official Symbol: PRAF2
• NCBI Official Synonym Symbols: JM4
• NCBI Protein Information: PRA1 family protein 2
• UniProt Protein Name: PRA1 family protein 2
• Protein Family: PRA1 family protein
• UniProt Gene Name: PRAF2
• UniProt Entry Name: PRAF2_HUMAN

Uniprot Description

PRAF2: May be involved in ER/Golgi transport and vesicular traffic. Plays a proapoptic role in cerulenin-induced neuroblastoma apoptosis. Belongs to the PRA1 family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: Xp11.23

Cellular Component: endosome membrane; integral to membrane

Biological Process: L-glutamate transport; protein transport