Western Blot (WB)
(Western blot analysis of lysate from SH-SY5Y cell line,using JM4 Antibody(MBS9211425). MBS9211425 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug.)
Rabbit JM4 Polyclonal Antibody | anti-PRAF2 antibody
JM4 Antibody
Gene Names
PRAF2; JM4
Reactivity
Human, mouse, rat
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
JM4; Polyclonal Antibody; JM4 Antibody; PRA1 family protein 2; PRAF2; anti-PRAF2 antibody
Host
Rabbit
Reactivity
Human, mouse, rat
Clonality
Polyclonal
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Positions
152-178
Sequence Length
178
Applicable Applications for anti-PRAF2 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Western blot analysis of lysate from SH-SY5Y cell line,using JM4 Antibody(MBS9211425). MBS9211425 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug.)
Western Blot (WB)
(Western blot analysis of lysate from SH-SY5Y cell line,using JM4 Antibody(MBS9211425). MBS9211425 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug.)
Western Blot (WB)
(Western blot analysis of extracts from A549 cells (Lane 2), using JM4 Antibody. The lane on the left is treated with systhesized peptide.)
Western Blot (WB)
(Western blot analysis of extracts from A549 cells (Lane 2), using JM4 Antibody. The lane on the left is treated with systhesized peptide.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human brain tissue using JM4 antibody (#MBS9211425).)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human brain tissue using JM4 antibody (#MBS9211425).)
Related Product Information for anti-PRAF2 antibody
May be involved in ER/Golgi transport and vesicular traffic. Plays a proapoptic role in cerulenin-induced neuroblastoma apoptosis.
Product Categories/Family for anti-PRAF2 antibody
References
Strom T.M.,et al.Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases.
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Schweneker M.,et al.FEBS Lett. 579:1751-1758(2005).
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Schweneker M.,et al.FEBS Lett. 579:1751-1758(2005).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19258
NCBI Official Full Name
PRA1 family protein 2
NCBI Official Synonym Full Names
PRA1 domain family, member 2
NCBI Official Symbol
PRAF2
NCBI Official Synonym Symbols
JM4
NCBI Protein Information
PRA1 family protein 2
UniProt Protein Name
PRA1 family protein 2
Protein Family
UniProt Gene Name
PRAF2
UniProt Entry Name
PRAF2_HUMAN
Uniprot Description
PRAF2: May be involved in ER/Golgi transport and vesicular traffic. Plays a proapoptic role in cerulenin-induced neuroblastoma apoptosis. Belongs to the PRA1 family.
Protein type: Membrane protein, multi-pass; Membrane protein, integral
Chromosomal Location of Human Ortholog: Xp11.23
Cellular Component: integral to membrane; endosome membrane
Biological Process: protein transport; L-glutamate transport
Research Articles on PRAF2
Similar Products
Product Notes
The PRAF2 praf2 (Catalog #AAA9211425) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 152-178. The JM4 Antibody reacts with Human, mouse, rat and may cross-react with other species as described in the data sheet. AAA Biotech's JM4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the PRAF2 praf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JM4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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