Rabbit anti-Human, Mouse POLD3 Polyclonal Antibody | anti-POLD3 antibody

POLD3, CT (POLD3, KIAA0039, DNA polymerase delta subunit 3, DNA polymerase delta subunit p66) (PE)

Cat. Num. AAA6335586

• Gene Names: POLD3; P66; P68
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: POLD3; Polyclonal Antibody; CT (POLD3; KIAA0039; DNA polymerase delta subunit 3; DNA polymerase delta subunit p66) (PE); DNA polymerase delta subunit p66; anti-POLD3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-POLD3 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: POLD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 415-443 amino acids from the C-terminal region of human POLD3.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(POLD3 Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the POLD3 antibody detected the POLD3 protein (arrow).)

Western Blot (WB)

(POLD3 Antibody (C-term) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the POLD3 antibody detected the POLD3 protein (arrow).)

Product Categories/Family for anti-POLD3 antibody

Antibodies; KIAA; orf

References

Michiels, S., et al. Carcinogenesis 30(5):763-768(2009). Baranovskiy, A.G., et al. Cell Cycle 7(19):3026-3036(2008). Baranovskiy, A.G., et al. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 64 (PT 9), 822-824 (2008): Lemmens, L., et al. Biochem. Biophys. Res. Commun. 367(2):264-270(2008). Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 38492356
• NCBI GeneID: 10714
• NCBI Accession #: NP_006582.1
• NCBI GenBank Nucleotide #: NM_006591.2
• UniProt Accession #: Q15054; B7ZAI6
• Molecular Weight: 51,400 Da
• NCBI Official Full Name: DNA polymerase delta subunit 3
• NCBI Official Synonym Full Names: polymerase (DNA-directed), delta 3, accessory subunit
• NCBI Official Symbol: POLD3
• NCBI Official Synonym Symbols: P66; P68
• NCBI Protein Information: DNA polymerase delta subunit 3; Pol delta C subunit (p66); DNA polymerase delta subunit p66
• UniProt Protein Name: DNA polymerase delta subunit 3
• UniProt Gene Name: POLD3
• UniProt Synonym Gene Names: KIAA0039
• UniProt Entry Name: DPOD3_HUMAN

NCBI Description

This gene encodes the 66-kDa subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. The encoded protein plays a role in regulating the activity of DNA polymerase delta through interactions with other subunits and the processivity cofactor proliferating cell nuclear antigen (PCNA). Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Mar 2012]

Uniprot Description

POLD3: Required for optimal DNA polymerase delta activity.

Protein type: Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine; DNA replication

Chromosomal Location of Human Ortholog: 11q14

Cellular Component: nucleoplasm; delta DNA polymerase complex; mitochondrion; nucleus

Molecular Function: protein binding; DNA-directed DNA polymerase activity

Biological Process: telomere maintenance via semi-conservative replication; DNA synthesis during DNA repair; mismatch repair; base-excision repair; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; mitotic cell cycle; nucleotide-excision repair, DNA gap filling; DNA strand elongation during DNA replication; DNA repair; telomere maintenance