Human Dystroglycan ELISA Kit | DAG1 elisa kit

Human Dystroglycan (DAG1) ELISA Kit

Cat. Num. AAA2606284

• Gene Names: DAG1; A3a; DAG; AGRNR; 156DAG; MDDGA9; MDDGC7; MDDGC9
• Reactivity: Human
• Synonyms: Dystroglycan; Human Dystroglycan (DAG1) ELISA Kit; DAG1 elisa kit

• Reactivity: Human
• Specificity: No cross-reaction with other factors.
• Sequence Length: 895

• Samples: Serum, plasma or cell culture supernatant and organizations in the natural and recombinant DAG1 concentration
• Assay Type: Sandwich
• Detection Range: 20 ng/ml-0.312 ng/ml
• Sensitivity: 0.06 ng/ml.
• Intra-assay Precision: ? 8%
• Inter-assay Precision: ? 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DAG1 elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human DAG1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 398026
• NCBI GeneID: 1605
• Molecular Weight: 97,441 Da
• NCBI Official Full Name: dystroglycan
• NCBI Official Synonym Full Names: dystroglycan 1
• NCBI Official Symbol: DAG1
• NCBI Official Synonym Symbols: A3a; DAG; AGRNR; 156DAG; MDDGA9; MDDGC7; MDDGC9
• NCBI Protein Information: dystroglycan
• UniProt Protein Name: Dystroglycan
• Protein Family: Dystroglycan
• UniProt Gene Name: DAG1
• UniProt Synonym Gene Names: Alpha-DG; Beta-DG
• UniProt Entry Name: DAG1_HUMAN

NCBI Description

This gene encodes dystroglycan, a central component of dystrophin-glycoprotein complex that links the extracellular matrix and the cytoskeleton in the skeletal muscle. The encoded preproprotein undergoes O- and N-glycosylation, and proteolytic processing to generate alpha and beta subunits. Certain mutations in this gene are known to cause distinct forms of muscular dystrophy. Alternative splicing results in multiple transcript variants, all encoding the same protein. [provided by RefSeq, Nov 2015]

Uniprot Description

DAG1: a cytoskeletal protein that functions as a laminin receptor. Provides a linkage between the subsarcolemmal cytoskeleton and the extracellular matrix. May be involved in autosomal recessive muscular dystrophies. Its dramatic reduction in Duchenne muscular dystrophy leads to a loss of linkage between the sarcolemma and extracellular matrix, rendering muscle fibers more susceptible to necrosis. Dystroglycan also functions as dual receptor for agrin and laminin-2 in the Schwann cell membrane. Binds to several types of arenaviruses. Is a target for the entry of Mycobacterium leprae into peripheral nerve Schwann cells. The muscle and nonmuscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence.

Protein type: Membrane protein, integral; Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3p21

Cellular Component: basement membrane; basolateral plasma membrane; cell-cell adherens junction; costamere; cytoplasm; cytoskeleton; dystroglycan complex; dystrophin-associated glycoprotein complex; extracellular region; extracellular space; filopodium; focal adhesion; integral to membrane; lamellipodium; lipid raft; nucleoplasm; plasma membrane; postsynaptic membrane; sarcolemma

Molecular Function: actin binding; alpha-actinin binding; calcium ion binding; laminin-1 binding; protein binding; protein complex binding; SH2 domain binding; structural constituent of muscle; tubulin binding; vinculin binding; viral receptor activity

Biological Process: calcium-dependent cell-matrix adhesion; cytoskeletal anchoring; entry of virus into host cell; extracellular matrix organization and biogenesis; membrane protein ectodomain proteolysis; morphogenesis of an epithelial sheet; myelination in the peripheral nervous system; negative regulation of cell migration; negative regulation of MAPKKK cascade; negative regulation of protein kinase B signaling cascade; nerve maturation; NLS-bearing substrate import into nucleus; regulation of gastrulation; response to peptide hormone stimulus; shape changes of embryonic cells; virus-host interaction

Disease: Muscular Dystrophy-dystroglycanopathy (congenital With Brain And Eye Anomalies), Type A, 9; Muscular Dystrophy-dystroglycanopathy (limb-girdle), Type C, 9