Western Blot (WB)
(Anti-PPP4C rabbit polyclonal antibody at 1:500 dilution Lane A: Hela Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:35 kDa Observed band size:35 kDa)
Rabbit anti-Human PPP4C Polyclonal Antibody | anti-PPP4C antibody
Anti-PPP4C Antibody, Rabbit Polyclonal
Gene Names
PPP4C; PP4; PPX; PP-X; PP4C; PPH3; PPP4
Reactivity
Human
Applications
Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
PPP4C; Polyclonal Antibody; Anti-PPP4C Antibody; Rabbit Polyclonal; PPP4C Antibody; Rabbit PAb; Antigen Affinity Purified; protein phosphatase 4; catalytic subunit; Anti-PP-X Antibody; Anti-PP4 Antibody; Anti-PP4C Antibody; Anti-PPH3 Antibody; Anti-PPP4 Antibody; Anti-PPX Antibody; anti-PPP4C antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human PPP4C
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-PPP4C antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthetic peptide corresponding to the center region of the Human PPP4C
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human PPP4C, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-PPP4C rabbit polyclonal antibody at 1:500 dilution Lane A: Hela Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:35 kDa Observed band size:35 kDa)
Western Blot (WB)
(Anti-PPP4C rabbit polyclonal antibody at 1:500 dilution Lane A: Hela Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:35 kDa Observed band size:35 kDa)
Related Product Information for anti-PPP4C antibody
The mammalian orthologue of Pph3p is the protein serine/threonine phosphatase Ppp4c, which is found in high molecular mass complexes bound to a regulatory subunit R2. Protein phosphates 4 (PP4), encoded by the ppp4c gene, is a ubiquitously expressed phosphatase that has been implicated in the regulation of cytokine signaling and lymphocyte survival; recent reports suggest that PP4 may be involved in pre-TCR signaling and B cell development. Ppp4c is a critical positive regulator of BMP/Smad signaling during embryonic dorsoventral pattern formation in zebrafish. Ppp4c is required for the organization of the actin cytoskeleton at the leading edge of human cells during migration. The active forms of the RhoGTPases, Rac1, and Cdc42 are substantially decreased in the presence and absence of growth factor in Ppp4c depleted cells, implicating Ppp4c in the regulation of these GTPases.
Product Categories/Family for anti-PPP4C antibody
References
Martin-Granados C, et al. (2008) Depletion of protein phosphatase 4 in human cells reveals essential roles in centrosome maturation, cell migration and the regulation of rho gtpases. Int J Biochem Cell Biol 40 (10): 2315-2332.Liao FH, et al. (2014) Protein phosphatase 4 is an essential positive regulator for treg development, function, and protective gut immunity. Cell Biosci 4 25.Hastie CJ, et al. (2006) The saccharomyces cerevisiae orthologue of the human protein phosphatase 4 core regulatory subunit r2 confers resistance to the anticancer drug cisplatin. Febs j 273 (14): 3322-3334.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
307
NCBI Official Full Name
Serine/threonine-protein phosphatase 4 catalytic subunit
NCBI Official Synonym Full Names
protein phosphatase 4, catalytic subunit
NCBI Official Symbol
PPP4C
NCBI Official Synonym Symbols
PP4; PPX; PP-X; PP4C; PPH3; PPP4
NCBI Protein Information
serine/threonine-protein phosphatase 4 catalytic subunit; protein phosphatase X, catalytic subunit; protein phosphatase 4 (formerly X), catalytic subunit
UniProt Protein Name
Serine/threonine-protein phosphatase 4 catalytic subunit
UniProt Gene Name
PPP4C
UniProt Synonym Gene Names
PPP4; PPX; PP4C; Pp4; PP-X
UniProt Entry Name
PP4C_HUMAN
Uniprot Description
PPP4C: a Ser/Thr phosphatase. A positive regulator of HPK1 and the HPK1-JNK signaling pathway. Mediates TNF-alpha-induced degradation of IRS-4 and plays a role in NF-kappaB p65 Thr dephosphorylation. Holoenzyme consists at least of a catalytic subunit PPP4C and a regulatory subunit PPP4R1.
Protein type: EC 3.1.3.16; Protein phosphatase, Ser/Thr (non-receptor)
Chromosomal Location of Human Ortholog: 16p11.2
Cellular Component: nucleoplasm; cytoplasm; plasma membrane; microtubule organizing center; nucleus
Molecular Function: NF-kappaB-inducing kinase activity; protein binding; metal ion binding; protein serine/threonine phosphatase activity
Biological Process: dephosphorylation
Research Articles on PPP4C
Similar Products
Product Notes
The PPP4C ppp4c (Catalog #AAA8103188) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PPP4C Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PPP4C can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the PPP4C ppp4c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPP4C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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