Goat PPP2R5E Polyclonal Antibody | anti-PPP2R5E antibody

Goat anti-PPP2R5E Antibody

Cat. Num. AAA420815

• Reactivity: Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: PPP2R5E; Polyclonal Antibody; Goat anti-PPP2R5E Antibody; protein phosphatase 2; regulatory subunit B (B56); epsilon isoform; PP2A; B subunit; B' epsilon isoform; R5 epsilon isoform; B56 epsilon isoform; PR61 epsilon isoform; Serine/threonine protein ph; PPP2R5E antibody; epsilon isoform antibody; B\1 epsilon isoform antibody; R5 epsilon isoform antibody; B56 epsilon isoform antibody; PR61 epsilon isoform antibody; Serine/threonine protein phosphatase 2A; 56 kDa regulatory subunit; regulatory subunit B\1; R5 epsilon antibody; epsilon isoform of regulatory subunit B56; protein phosphatase 2A antibody; serine/threonine protein phosphatase 2A; epsilon antibody; anti-PPP2R5E antibody

• Host: Goat
• Reactivity: Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: LKRGLRRDGIIPT
• Sequence Length: 467

• Applicable Applications for anti-PPP2R5E antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 16000.; Western Blot: Approx 50kDa band observed in Human (Cerebellum), Mouse and Rat Brain lysates (calculated MW of 54.7kDa according to Human NP_001269108.1 and Mouse NP_036154.1 and 50.4kDa according to Rat NP_001100210.1). Recommended concentration: 0.1-0.3ug/ml.
• Immunogen: Peptide with sequence C-LKRGLRRDGIIPT, from the C Terminus of the protein sequence according to NP_006237.1.
• Epitope: C Terminus
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.1ug/ml) staining of Human Cerebellum (A), Mouse (B) and Rat (C) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 5453956
• NCBI GeneID: 5529
• NCBI Accession #: NP_006237.1
• NCBI GenBank Nucleotide #: NM_006246.3
• Molecular Weight: 46,180 Da
• NCBI Official Full Name: serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilon isoform isoform a
• NCBI Official Synonym Full Names: protein phosphatase 2 regulatory subunit B'epsilon
• NCBI Official Symbol: PPP2R5E
• NCBI Protein Information: serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilon isoform
• UniProt Protein Name: Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilon isoform
• Protein Family: Serine/threonine-protein phosphatase
• UniProt Gene Name: PPP2R5E
• UniProt Entry Name: 2A5E_HUMAN

NCBI Description

The protein encoded by this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an epsilon isoform of the regulatory subunit B56 subfamily. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Aug 2013]

Uniprot Description

PPP2R5E: The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. Belongs to the phosphatase 2A regulatory subunit B56 family.

Protein type: Protein phosphatase, regulatory subunit; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 14q23.1

Cellular Component: cytoplasm; protein phosphatase type 2A complex

Molecular Function: protein binding; protein phosphatase type 2A regulator activity

Biological Process: regulation of protein phosphatase type 2A activity; signal transduction