Typical Testing Data/Standard Curve (for reference only)
Mouse Kallikrein 10 ELISA Kit | KLK10 elisa kit
Mouse Kallikrein 10 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: KLH ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-KLH antibody and an KLH-HRP conjugate. The assay sample and buffer are incubated together with KLH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the KLH concentration since KLH from samples and KLH-HRP conjugate compete for the anti-KLH antibody binding site. Since the number of sites is limited, as more sites are occupied by KLH from the sample, fewer sites are left to bind KLH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KLH concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its encoded protein is secreted and may play a role in suppression of tumorigenesis in breast and prostate cancers. Alternate splicing of this gene results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]
Uniprot Description
KLK10: Has a tumor-suppressor role for NES1 in breast and prostate cancer. Belongs to the peptidase S1 family. Kallikrein subfamily.
Protein type: Tumor suppressor; Protease; Secreted, signal peptide; Secreted; EC 3.4.21.-
Chromosomal Location of Human Ortholog: 19q13
Cellular Component: extracellular region
Molecular Function: serine-type peptidase activity; serine-type endopeptidase activity
Biological Process: cell cycle; proteolysis
Research Articles on KLK10
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Product Notes
The Mouse KLK10 klk10 (Catalog #AAA722747) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA722747 ELISA Kit recognizes Mouse KLK10. It is sometimes possible for the material contained within the vial of "Kallikrein 10, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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