Western Blot (WB)
(Western Blot analysis of PPP1R1A on Rat brain lysates using anti-PPP1R1A antibody at 1/1,000 dilution.)
Rabbit PPP1R1A Polyclonal Antibody | anti-PPP1R1A antibody
PPP1R1A Polyclonal Antibody
Gene Names
PPP1R1A; I1; IPP1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
PPP1R1A; Polyclonal Antibody; PPP1R1A Polyclonal Antibody; I-1; IPP-1; I 1; I1; Inhibitor 1; IPP 1; IPP1; Ppp1r1a; PPR1A; Protein phosphatase 1 regulatory (inhibitor) subunit 1A; Protein phosphatase 1 regulatory subunit 1A; Protein phosphatase inhibitor 1; anti-PPP1R1A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
PPP1R1A polyclonal antibody detects endogenous levels of PPP1R1A protein.
Purity/Purification
ProA affinity purified
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
171
Applicable Applications for anti-PPP1R1A antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1,000
ICC: 1:50-1:200
IHC: 1:100-1:500
FC/FACS: 1:50-1:100
ICC: 1:50-1:200
IHC: 1:100-1:500
FC/FACS: 1:50-1:100
Immunogen
recombinant protein
Preparation and Storage
Store at 4 degree C after thawing.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of PPP1R1A on Rat brain lysates using anti-PPP1R1A antibody at 1/1,000 dilution.)
Western Blot (WB)
(Western Blot analysis of PPP1R1A on Rat brain lysates using anti-PPP1R1A antibody at 1/1,000 dilution.)
Immunocytochemistry (ICC)
(ICC staining PPP1R1A in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining PPP1R1A in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Related Product Information for anti-PPP1R1A antibody
The inhibitor of protein phosphatase 1 (IPP-1, I-1) plays a role in regulating the phosphorylation of other proteins, and is itself phosphorylated by a cyclic AMP-dependent protein kinase. IPP-1 is present in skeletal muscles and in distinct neuronal systems of the brain. The localization and expression of IPP-1 suggests that it may play discrete roles in certain regions and developing stages of the brain, independent of the regulation of protein phosphatase type 1 (PP-1). PP-1 binds to both phosphorylated and dephosphorylated IPP-1. Conversion of PP-1 to an Mn2+-dependent state appears to play a role in its regulation by IPP-1. IPP-1 attenuates the activity of glycogen phosphorylase and is thought to play an important role in the hormonal control of glycogen metabolism.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27kDa
NCBI Official Full Name
protein phosphatase 1 regulatory subunit 1A
NCBI Official Synonym Full Names
protein phosphatase 1 regulatory inhibitor subunit 1A
NCBI Official Symbol
PPP1R1A
NCBI Official Synonym Symbols
I1; IPP1
NCBI Protein Information
protein phosphatase 1 regulatory subunit 1A
UniProt Protein Name
Protein phosphatase 1 regulatory subunit 1A
Protein Family
UniProt Gene Name
PPP1R1A
UniProt Synonym Gene Names
IPP1; I-1; IPP-1
UniProt Entry Name
PPR1A_HUMAN
Uniprot Description
PPP1R1A: protein phosphatase 1, regulatory (inhibitor) subunit 1A. I-1 activation may impose cAMP control over proteins that are not directly phosphorylated by PKA. Following a rise in intracellular calcium, I-1 is inactivated by calcineurin (or PP2B). Does not inhibit type-2 phosphatases.
Protein type: Protein phosphatase, regulatory subunit; Inhibitor
Chromosomal Location of Human Ortholog: 12q13.2
Cellular Component: extracellular space; cytoplasm
Molecular Function: protein binding; type 1 serine/threonine specific protein phosphatase inhibitor activity
Biological Process: glycogen metabolic process; negative regulation of protein kinase activity
Research Articles on PPP1R1A
Similar Products
Product Notes
The PPP1R1A ppp1r1a (Catalog #AAA3013287) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PPP1R1A Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PPP1R1A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:1,000 ICC: 1:50-1:200 IHC: 1:100-1:500 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the PPP1R1A ppp1r1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPP1R1A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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