Goat PPP1R12A / MYPT1 Polyclonal Antibody | anti-PPP1R12A antibody

Goat Polyclonal to Human PPP1R12A / MYPT1

Cat. Num. AAA244384

• Gene Names: PPP1R12A; MBS; M130; MYPT1
• Reactivity: Bat, Gibbon, Bovine, Dog, Gorilla, Horse, Human, Monkey, Mouse, Orangutan, Rat; Predicted Reactivity: Hamster (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: PPP1R12A / MYPT1; Polyclonal Antibody; Goat Polyclonal to Human PPP1R12A / MYPT1; Anti-PPP1R12A / MYPT1 Antibody IHC-plus; PPP1R12A; M130; MYPT1; MBS; Myosin binding subunit; Human PPP1R12A; anti-PPP1R12A antibody

• Host: Goat
• Reactivity: Bat, Gibbon, Bovine, Dog, Gorilla, Horse, Human, Monkey, Mouse, Orangutan, Rat; Predicted Reactivity: Hamster (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human PPP1R12A / MYPT1.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 1030

• Applicable Applications for anti-PPP1R12A antibody: Immunohistochemistry (IHC - Paraffin)
• Application Notes: IHC-P (3.75 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human PPP1R12A / MYPT1. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Dog, Bat, Horse, Opossum, Platypus (100%); Hamster (93%); Rabbit (86%).
• Immunogen: PPP1R12A / MYPT1 antibody was raised against synthetic peptide from human PPP1R12A / MYPT1. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Dog, Bat, Horse, Opossum, Platypus (100%); Hamster (93%); Rabbit (86%).
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-PPP1R12A / MYPT1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Related Product Information for anti-PPP1R12A antibody

Myosin phosphatase target subunit 1 (MYPT1), which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells.

NCBI and Uniprot Product Information

• NCBI GI #: 4505317
• NCBI GeneID: 4659
• NCBI Accession #: NP_002471.1
• NCBI GenBank Nucleotide #: NM_002480.2
• UniProt Accession #: O14974; Q2NKL4; Q569H0; Q86WU3; Q8NFR6; Q9BYH0; B4DZ09; F8VWB4
• Molecular Weight: 105,641 Da
• NCBI Official Full Name: protein phosphatase 1 regulatory subunit 12A isoform a
• NCBI Official Synonym Full Names: protein phosphatase 1, regulatory subunit 12A
• NCBI Official Symbol: PPP1R12A
• NCBI Official Synonym Symbols: MBS; M130; MYPT1
• NCBI Protein Information: protein phosphatase 1 regulatory subunit 12A; myosin binding subunit; myosin phosphatase, target subunit 1; myosin phosphatase-targeting subunit 1; protein phosphatase myosin-binding subunit; protein phosphatase 1, regulatory (inhibitor) subunit 12A
• UniProt Protein Name: Protein phosphatase 1 regulatory subunit 12A
• Protein Family: Protein phosphatase 1 regulatory
• UniProt Gene Name: PPP1R12A
• UniProt Synonym Gene Names: MBS; MYPT1; Myosin phosphatase target subunit 1
• UniProt Entry Name: MYPT1_HUMAN

NCBI Description

Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009]

Uniprot Description

MYPT1: myosin phosphatase target subunit 1, a regulatory subunit of protein phosphatase 1. Also called the myosin-binding subunit of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. Four splice-variant isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Protein phosphatase, regulatory subunit

Chromosomal Location of Human Ortholog: 12q15-q21

Cellular Component: nucleoplasm; kinetochore; centrosome; focal adhesion; contractile fiber; cytoplasm; actin cytoskeleton

Molecular Function: enzyme inhibitor activity; signal transducer activity; protein binding; phosphatase regulator activity; protein kinase binding

Biological Process: regulation of cell adhesion; mitosis; centrosome organization and biogenesis; regulation of nucleocytoplasmic transport; negative regulation of catalytic activity; positive regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; signal transduction; G2/M transition of mitotic cell cycle; protein amino acid dephosphorylation