Rabbit anti-Human PPP1CB Polyclonal Antibody | anti-PPP1CB antibody

PPP1CB Antibody

Cat. Num. AAA7052281

• Gene Names: PPP1CB; PP1B; PP-1B; PPP1CD; PP1beta; HEL-S-80p
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: >95%, Protein G purified
• Synonyms: PPP1CB; Polyclonal Antibody; PPP1CB Antibody; Serine/threonine-protein phosphatase PP1-beta catalytic subunit; PP-1B; PPP1CD; anti-PPP1CB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 327

• Applicable Applications for anti-PPP1CB antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:5000; IHC: 1:20-1:200
• Immunogen: Recombinant Human Serine/threonine-protein phosphatase PP1-beta catalytic subunit protein (2-327AA)
• Conjugation: Non-conjugated
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: Raji whole cell lysate,HEK293 whole cell lysate,A375 whole cell lysateAll lanes: Ppp1cb antibody at 3.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 38 KDaObserved band size: 38 KDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human cervical cancer using MBS7052281 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human ovarian cancer using MBS7052281 at dilution of 1:100)

Related Product Information for anti-PPP1CB antibody

Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase (PP1) is essential for cell division, it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. Dephosphorylates the 'Ser-418' residue of FOXP3 in regulatory T-cells (Treg) from patients with rheumatoid arthritis, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208).

NCBI and Uniprot Product Information

• NCBI GI #: 4506005
• NCBI GeneID: 5500
• NCBI Accession #: NP_002700.1
• NCBI GenBank Nucleotide #: NM_002709.2
• UniProt Accession #: P62140; P37140; Q5U087; Q6FG45; B2R5V4; D6W565
• Molecular Weight: 37,187 Da
• NCBI Official Full Name: serine/threonine-protein phosphatase PP1-beta catalytic subunit isoform 1
• NCBI Official Synonym Full Names: protein phosphatase 1 catalytic subunit beta
• NCBI Official Symbol: PPP1CB
• NCBI Official Synonym Symbols: PP1B; PP-1B; PPP1CD; PP1beta; HEL-S-80p
• NCBI Protein Information: serine/threonine-protein phosphatase PP1-beta catalytic subunit
• UniProt Protein Name: Serine/threonine-protein phosphatase PP1-beta catalytic subunit
• Protein Family: Serine/threonine-protein phosphatase
• UniProt Gene Name: PPP1CB
• UniProt Synonym Gene Names: PP-1B; PPP1CD

NCBI Description

The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Mouse studies suggest that PP1 functions as a suppressor of learning and memory. Two alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

PPP1CB: is a catalytic subunit of protein phosphatase 1 (PP1), a ubiquitous cytosolic serine-threonine phosphatase. Composed a catalytic subunit (PPP1CA, PPP1CB or PPP1CC) which is folded into its native form by inhibitor 2 and glycogen synthetase kinase 3, and then complexed to one or several targeting (or regulatory) subunits: PPP1R12A and PPP1R12B mediate binding to myosin; PPP1R3A, PPP1R3B, PPP1R3C and PPP1R3D mediate binding to glycogen; and PPP1R15A and PPP1R15B mediate binding to EIF2S1. PP1 is essential for cell division, plays critical roles in many cellular processes including glycogen metabolism, muscle contractility and protein synthesis, and is involved in the regulation of ionic conductances and long-term synaptic plasticity. Inhibits the synthesis of proteins involved in synaptic plasticity. Binds to the transcription factor cyclic AMP-dependent response element binding (CREB) and dephosphorylates it. May regulate chromatin condensation through regulation of histone H3 phosphorylation. Differentially expressed in gastric cancer. May participate in the neurodegenerative progress in Alzheimer's disease. Down-regulated in lung squamous cell carcinoma.

Protein type: EC 3.1.3.16; EC 3.1.3.53; Motility/polarity/chemotaxis; Nucleolus; Protein phosphatase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 2p23.2

Cellular Component: cytosol; focal adhesion; nuclear chromosome, telomeric region; nucleoplasm; nucleus

Molecular Function: myosin phosphatase activity; myosin-light-chain-phosphatase activity; phosphoric monoester hydrolase activity; protein binding; protein kinase binding

Biological Process: circadian regulation of gene expression; entrainment of circadian clock by photoperiod; G2/M transition of mitotic cell cycle; protein amino acid dephosphorylation; regulation of cell adhesion; regulation of circadian rhythm