Western Blot (WB)
(Western Blot analysis of PPM1K expression in transfected 293T cell line by PPM1K polyclonal antibody. Lane 1: PPM1K transfected lysate (40.92kD). Lane 2: Non-transfected lysate.)
Mouse anti-Human PPM1K Polyclonal Antibody | anti-PPM1K antibody
PPM1K (Protein Phosphatase 1K, Mitochondrial, PP2C Domain-containing Protein Phosphatase 1K, PP2C-like Mitochondrial Protein, PP2C-type Mitochondrial Phosphoprotein Phosphatase, PTMP, Protein Phosphatase 2C Isoform kappa, PP2C-kappa, PP2CM, DKFZp667B084,
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Western Blot (WB)
(Western Blot analysis of PPM1K expression in transfected 293T cell line by PPM1K polyclonal antibody. Lane 1: PPM1K transfected lysate (40.92kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of PPM1K expression in transfected 293T cell line by PPM1K polyclonal antibody. Lane 1: PPM1K transfected lysate (40.92kD). Lane 2: Non-transfected lysate.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the PPM family of Mn2+/Mg2+-dependent protein phosphatases. The encoded protein, essential for cell survival and development, is targeted to the mitochondria where it plays a key role in regulation of the mitochondrial permeability transition pore. [provided by RefSeq, Sep 2012]
Uniprot Description
Function: Regulates the mitochondrial permeability transition pore and is essential for cellular survival and development. Ref.1
Catalytic activity: A phosphoprotein + H2O = a protein + phosphate.
Cofactor: Binds 1 magnesium or manganese ion per subunit.
Subcellular location: Mitochondrion matrix Ref.1 Ref.3.
Involvement in disease: Maple syrup urine disease, mild variant (MSUDMV) [MIM:615135]: A mild form of maple syrup urine disease, a metabolic disorder due to an enzyme defect in the catabolic pathway of the branched-chain amino acids leucine, isoleucine, and valine. Accumulation of these 3 amino acids and their corresponding keto acids leads to encephalopathy and progressive neurodegeneration. Clinical features include mental and physical retardation, feeding problems, and a maple syrup odor to the urine. The keto acids of the branched-chain amino acids are present in the urine. If untreated, maple syrup urine disease can lead to seizures, coma, and death. The disease is often classified by its pattern of signs and symptoms. The most common and severe form of the disease is the classic type, which becomes apparent soon after birth. Variant forms of the disorder become apparent later in infancy or childhood and are typically milder, but they still involve developmental delay and other medical problems if not treated. MSUDMV is characterized by increased plasma levels of branched-chain amino acids (BCAA) apparent at birth. Treatment with a low-protein diet free of BCAA can result in normal psychomotor development and lack of metabolic episodes.Note: The gene represented in this entry is involved in disease pathogenesis. Ref.10
Sequence similarities: Belongs to the PP2C family.Contains 1 PP2C-like domain.
Caution: Ref.11 has crystallized PPM1K in the presence of magnesium ions. However, Ref.3 reported that no activity toward p-nitrophenylphosphate was seen in the absence of manganese ions and magnesium could not substitute for manganese.
Biophysicochemical propertiesKinetic parameters:Half maximal activity toward p-nitrophenylphosphate achieved with 3.7 mM of manganese ions.KM=10.7 mM for p-nitrophenylphosphate Ref.3Vmax=3.6 µmol/min/mg enzyme toward p-nitrophenylphosphate (at 30 degrees Celsius)Vmax=4 nmol/min/mg enzyme toward branched-chain alpha-ketoacid dehydrogenase complex (at 37 degrees Celsius)
Research Articles on PPM1K
Similar Products
Product Notes
The PPM1K ppm1k (Catalog #AAA649272) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PPM1K (Protein Phosphatase 1K, Mitochondrial, PP2C Domain-containing Protein Phosphatase 1K, PP2C-like Mitochondrial Protein, PP2C-type Mitochondrial Phosphoprotein Phosphatase, PTMP, Protein Phosphatase 2C Isoform kappa, PP2C-kappa, PP2CM, DKFZp667B084, reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PPM1K can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Researchers should empirically determine the suitability of the PPM1K ppm1k for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MSTAALITLV RSGGNQVRRR VLLSSRLLQD DRRVTPTCHS STSEPRCSRF DPDGSGSPAT WDNFGIWDNR IDEPILLPPS IKYGKPIPKI SLEKVGCASQ IGKRKENEDR FDFAQLTDEV LYFAVYDGHG GPAAADFCHT HMEKCIMDLL PKEKNLETLL TLAFLEIDKA FSSHARLSAD ATLLTSGTTA TVALLRDGIE LVVASVGDSR AILCRKGKPM KLTIDHTPER KDEKERIKKC GGFVAWNSLG QPHVNGRLAM TRSIGDLDLK TSGVIAEPET KRIKLHHADD SFLVLTTDGI NFMVNSQEIC DFVNQCHDPN EAAHAVTEQA IQYGTEDNST AVVVPFGAWG KYKNSEINFS FSRSFASSGR WA. It is sometimes possible for the material contained within the vial of "PPM1K, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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