Rabbit anti-Human PPM1F Polyclonal Antibody | anti-PPM1F antibody

PPM1F, CT (Protein Phosphatase 1F, Ca(2+)/Calmodulin-dependent Protein Kinase Phosphatase, CaM-kinase Phosphatase, CaMKPase, Partner of PIX 2, Protein Fem-2 Homolog, hFEM-2, KIAA0015, POPX2) (FITC)

Cat. Num. AAA6495469

• Gene Names: PPM1F; CAMKP; FEM-2; POPX2; hFEM-2; CaMKPase
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: PPM1F; Polyclonal Antibody; CT (Protein Phosphatase 1F; Ca(2+)/Calmodulin-dependent Protein Kinase Phosphatase; CaM-kinase Phosphatase; CaMKPase; Partner of PIX 2; Protein Fem-2 Homolog; hFEM-2; KIAA0015; POPX2) (FITC); anti-PPM1F antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PPM1F.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-PPM1F antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human PPM1F (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of PPM1F (arrow) using PPM1F Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PPM1F gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-PPM1F antibody

Antibodies; Enzymes; Phosphatase

References

1. Harvey, B.P., et al., J. Biol. Chem. 279(23):24889-24898 (2004). 2. Izmailova, E., et al., Nat. Med. 9(2):191-197 (2003). 3. Koh, C.G., et al., Curr. Biol. 12(4):317-321 (2002). 4. Tan, K.M., et al., J. Biol. Chem. 276(47):44193-44202 (2001).

NCBI and Uniprot Product Information

• NCBI GI #: 7661862
• NCBI GeneID: 9647
• NCBI Accession #: NP_055449
• NCBI GenBank Nucleotide #: NM_014634.3
• UniProt Accession #: P49593; Q96PM2; A8K6G3; B7Z2C3
• Molecular Weight: 38,518 Da
• NCBI Official Full Name: protein phosphatase 1F
• NCBI Official Synonym Full Names: protein phosphatase, Mg2+/Mn2+ dependent 1F
• NCBI Official Symbol: PPM1F
• NCBI Official Synonym Symbols: CAMKP; FEM-2; POPX2; hFEM-2; CaMKPase
• NCBI Protein Information: protein phosphatase 1F
• UniProt Protein Name: Protein phosphatase 1F
• Protein Family: Protein phosphatase
• UniProt Gene Name: PPM1F
• UniProt Synonym Gene Names: KIAA0015; POPX2; CaM-kinase phosphatase; CaMKPase; hFem-2
• UniProt Entry Name: PPM1F_HUMAN

NCBI Description

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

PPM1F: a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members can be negative regulators of cell stress response pathways. Interact with PAK-interacting exchange factor beta (ARHGEF7), blocking the effects PAK1, a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CaMK2-gamma) is one of the substrates of this phosphatase. Promotes apoptosis.

Protein type: Motility/polarity/chemotaxis; EC 3.1.3.16; Protein phosphatase, dual-specificity

Chromosomal Location of Human Ortholog: 22q11.22

Cellular Component: protein complex; perinuclear region of cytoplasm; cytosol

Molecular Function: protein binding; calmodulin-dependent protein phosphatase activity; metal ion binding; phosphoric monoester hydrolase activity; protein serine/threonine phosphatase activity

Biological Process: histone dephosphorylation; positive regulation of focal adhesion formation; positive regulation of chemotaxis; positive regulation of stress fiber formation; negative regulation of peptidyl-serine phosphorylation; positive regulation of caspase activity; negative regulation of protein kinase activity; negative regulation of transcription, DNA-dependent; positive regulation of growth