Western Blot (WB)
(Western blot analysis of PPM1E using HuvEc whole cell lysates)
Rabbit PPM1E Polyclonal Antibody | anti-PPM1E antibody
PPM1E Antibody
Gene Names
PPM1E; POPX1; PP2CH; caMKN; CaMKP-N
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
PPM1E; Polyclonal Antibody; PPM1E Antibody; Ca(2+)/calmodulin-dependent protein kinase phosphatase N; CaMKN; CaMKP-N; CaMKP-nucleus; KIAA1072; Nuclear calmodulin dependent protein kinase phosphatase; Partner of PIX 1; Partner of PIX-alpha; Partner of PIXA; POPX1; PP2CH; Ppm1e; PPM1E_HUMAN; Protein phosphatase 1E; Protein phosphatase Mg2+/Mn2+ dependent 1E; anti-PPM1E antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
PPM1E antibody detects endogenous levels of total PPM1E
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
755
Applicable Applications for anti-PPM1E antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:1000-3000
IHC: 1:200
IHC: 1:200
Immunogen
A synthesized peptide derived from human PPM1E
Subcellular Location
Nucleus. Cytoplasm. A truncated form, major form, with the C-terminal part missing, is mostly found in the cytoplasm and a little in the nucleus. The full length, minor form, is found in the nucleus.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Similarity
Pig (100%), Bovine (91%), Horse (100%), Sheep (91%), Rabbit (100%), Dog (100%), Chicken (91%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of PPM1E using HuvEc whole cell lysates)
Western Blot (WB)
(Western blot analysis of PPM1E using HuvEc whole cell lysates)
Immunohistochemistry (IHC)
(MBS9610098 at 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9610098 at 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-PPM1E antibody
Function: Protein phosphatase that inactivates multifunctional CaM kinases such as CAMK4 and CAMK2 (By similarity). Dephosphorylates and inactivates PAK. May play a role in the inhibition of actin fiber stress breakdown and in morphological changes driven by TNK2/CDC42. Dephosphorylates PRKAA2 (By similarity).
Subunit Structure: Heterotrimer. Interacts with PAX1 and ARHGEF6 (or ARHGEF7).
Similarity: Belongs to the PP2C family.
Subunit Structure: Heterotrimer. Interacts with PAX1 and ARHGEF6 (or ARHGEF7).
Similarity: Belongs to the PP2C family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 85 kDa
Predicted: 84 kDa
Predicted: 84 kDa
NCBI Official Full Name
protein phosphatase 1E
NCBI Official Synonym Full Names
protein phosphatase, Mg2+/Mn2+ dependent 1E
NCBI Official Symbol
PPM1E
NCBI Official Synonym Symbols
POPX1; PP2CH; caMKN; CaMKP-N
NCBI Protein Information
protein phosphatase 1E
UniProt Protein Name
Protein phosphatase 1E
Protein Family
UniProt Gene Name
PPM1E
UniProt Synonym Gene Names
CAMKN; KIAA1072; POPX1; CaMKP-N; CaMKN; Partner of PIXA
NCBI Description
This gene encodes a member of the PPM family of serine/threonine-protein phosphatases. The encoded protein is localized to the nucleus and dephosphorylates and inactivates multiple substrates including serine/threonine-protein kinase PAK 1, 5'-AMP-activated protein kinase (AMPK) and the multifunctional calcium/calmodulin-dependent protein kinases. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012]
Uniprot Description
Protein phosphatase that inactivates multifunctional CaM kinases such as CAMK4 and CAMK2 (). Dephosphorylates and inactivates PAK. May play a role in the inhibition of actin fiber stress breakdown and in morphological changes driven by TNK2/CDC42. Dephosphorylates PRKAA2 ().
Research Articles on PPM1E
Similar Products
Product Notes
The PPM1E ppm1e (Catalog #AAA9610098) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PPM1E Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PPM1E can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:1000-3000 IHC: 1:200. Researchers should empirically determine the suitability of the PPM1E ppm1e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPM1E, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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