CFP recombinant protein

CFP, 28-469aa, Human, His tag, E Coli (Denatured)

Cat. Num. AAA205664

• Gene Names: CFP; BFD; PFC; PFD; PROPERDIN
• Applications: SDS-Page
• Purity: > 80% by SDS-PAGE
• Synonyms: CFP; 28-469aa; Human; His tag; E Coli (Denatured); Complement factor properdin; BFD; PFC; PFD; PROPERDIN; CFP recombinant protein

• Host: E Coli
• Purity/Purification: > 80% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.4M urea, 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSDPVLCFT QYEESSGKCK GLLGGGVSVE DCCLNTAFAY QKRSGGLCQP CRSPRWSLWS TWAPCSVTCS EGSQLRYRRC VGWNGQCSGK VAPGTLEWQL QACEDQQCCP EMGGWSGWGP WEPCSVTCSK GTRTRRRACN HPAPKCGGHC PGQAQESEAC DTQQVCPTHG AWATWGPWTP CSASCHGGPH EPKETRSRKC SAPEPSQKPP GKPCPGLAYE QRRCTGLPPC PVAGGWGPWG PVSPCPVTCG LGQTMEQRTC NHPVPQHGGP FCAGDATRTH ICNTAVPCPV DGEWDSWGEW SPCIRRNMKS ISCQEIPGQQ SRGRTCRGRK FDGHRCAGQQ QDIRHCYSIQ HCPLKGSWSE WSTWGLCMPP CGPNPTRARQ RLCTPLLPKY PPTVSMVEGQ GEKNVTFWGR PLPRCEELQG QKLVVEEKRP CLHVPACKDP EEEEL
• Sequence Length: 469

• Applicable Applications for CFP recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for CFP recombinant protein

CFP is a plasma glycoprotein that positively regulates the alternative complement pathway of the innate immune system. This protein binds to many microbial surfaces and apoptotic cells and stabilizes the C3- and C5-convertase enzyme complexes in a feedback loop that ultimately leads to formation of the membrane attack complex and lysis of the target cell. Mutations in this gene result in two forms of properdin deficiency, which results in high susceptibility to meningococcal infections. Multiple alternatively spliced variants, encoding the same protein, have been identified. Recombinant human CFP protein, fused to His-tag at N-terminus, was expressed in E Coli.

Product Categories/Family for CFP recombinant protein

Immunology

References

Hartmann S., et al. (2000) J. Biol. Chem. 275:28569-28574; Liu T., et al. (2005) J. Proteome Res. 4:2070-2080;

NCBI and Uniprot Product Information

• NCBI GI #: 4505737
• NCBI GeneID: 5199
• NCBI Accession #: NP_002612
• NCBI GenBank Nucleotide #: NM_002621.2
• UniProt Accession #: P27918; O15134; O15135; O15136; O75826
• Molecular Weight: 50.9 kDa (465aa)
• NCBI Official Full Name: properdin
• NCBI Official Synonym Full Names: complement factor properdin
• NCBI Official Symbol: CFP
• NCBI Official Synonym Symbols: BFD; PFC; PFD; PROPERDIN
• NCBI Protein Information: properdin
• UniProt Protein Name: Properdin
• Protein Family: Properdin
• UniProt Gene Name: CFP
• UniProt Synonym Gene Names: PFC

NCBI Description

This gene encodes a plasma glycoprotein that positively regulates the alternative complement pathway of the innate immune system. This protein binds to many microbial surfaces and apoptotic cells and stabilizes the C3- and C5-convertase enzyme complexes in a feedback loop that ultimately leads to formation of the membrane attack complex and lysis of the target cell. Mutations in this gene result in two forms of properdin deficiency, which results in high susceptibility to meningococcal infections. Multiple alternatively spliced variants, encoding the same protein, have been identified.[provided by RefSeq, Feb 2009]

Uniprot Description

A positive regulator of the alternate pathway of complement. It binds to and stabilizes the C3- and C5-convertase enzyme complexes.