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Western Blot (WB) (Gel: 12%SDS-PAGE Lysate: 40 μ g Lane 1-7: PC3 cells, Raji cells, NIH/3T3 cells, K562 cells, hela cells, hepg2 cells, Jurkat cells Primary antibody: 1/400 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 5 seconds)

Rabbit anti-Human, Mouse PPIA Polyclonal Antibody | anti-PPIA antibody

PPIA Antibody

Gene Names
PPIA; CYPA; CYPH; HEL-S-69p
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Antigen Affinity Purification
Synonyms
PPIA; Polyclonal Antibody; PPIA Antibody; CYPA; CYPH; HEL-S-69p; anti-PPIA antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total PPIA protein.
Purity/Purification
Antigen Affinity Purification
Form/Format
Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
Concentration
1.8 mg/ml (varies by lot)
Sequence Length
105
Applicable Applications for anti-PPIA antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western Blot: 1:500-1:2000
Immunohistochemistry: 1:10-1:50
Immunogen
Fusion protein of human PPIA
Immunogen Type
Protein
Preparation and Storage
Store at -20 degree C

Western Blot (WB)

(Gel: 12%SDS-PAGE Lysate: 40 μ g Lane 1-7: PC3 cells, Raji cells, NIH/3T3 cells, K562 cells, hela cells, hepg2 cells, Jurkat cells Primary antibody: 1/400 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 5 seconds)

Western Blot (WB) (Gel: 12%SDS-PAGE Lysate: 40 μ g Lane 1-7: PC3 cells, Raji cells, NIH/3T3 cells, K562 cells, hela cells, hepg2 cells, Jurkat cells Primary antibody: 1/400 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 5 seconds)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue using at dilution 1/35,)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue using at dilution 1/35,)
Related Product Information for anti-PPIA antibody
This gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. The encoded protein is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. The protein can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions.
Product Categories/Family for anti-PPIA antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
11,418 Da
NCBI Official Full Name
peptidyl-prolyl cis-trans isomerase A isoform 2
NCBI Official Synonym Full Names
peptidylprolyl isomerase A
NCBI Official Symbol
PPIA
NCBI Official Synonym Symbols
CYPA; CYPH; HEL-S-69p
NCBI Protein Information
peptidyl-prolyl cis-trans isomerase A
UniProt Protein Name
Peptidyl-prolyl cis-trans isomerase A
UniProt Gene Name
PPIA
UniProt Synonym Gene Names
CYPA; PPIase A
UniProt Entry Name
PPIA_HUMAN

Uniprot Description

PPIA: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Interacts with HIV-1 Capsid protein. Binds cyclosporin A (CsA). CsA mediates some of its effects via an inhibitory action on PPIase. Belongs to the cyclophilin-type PPIase family. PPIase A subfamily.

Protein type: Cyclophilin; EC 5.2.1.8; Isomerase; RNA-binding

Chromosomal Location of Human Ortholog: 7p13

Cellular Component: cytosol; extracellular region; extracellular space; focal adhesion; membrane; nucleus

Molecular Function: peptidyl-prolyl cis-trans isomerase activity; protein binding; unfolded protein binding

Biological Process: entry into host cell; leukocyte migration; positive regulation of protein secretion; positive regulation of viral genome replication; protein folding; protein peptidyl-prolyl isomerization; regulation of viral genome replication; release of virus from host; RNA-dependent DNA replication; uncoating of virus; viral envelope fusion with host membrane; viral infectious cycle; virus assembly

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Product Notes

The PPIA ppia (Catalog #AAA9415417) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PPIA Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PPIA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: 1:500-1:2000 Immunohistochemistry: 1:10-1:50. Researchers should empirically determine the suitability of the PPIA ppia for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPIA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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