Rabbit anti-Human PPFIBP1 Polyclonal Antibody | anti-PPFIBP1 antibody

PPFIBP1, ID (PPFIBP1, KIAA1230, Liprin-beta-1, Protein tyrosine phosphatase receptor type f polypeptide-interacting protein-binding protein 1, hSGT2) (FITC)

Cat. Num. AAA6336228

• Gene Names: PPFIBP1; L2; SGT2; hSGT2; hSgt2p
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PPFIBP1; Polyclonal Antibody; ID (PPFIBP1; KIAA1230; Liprin-beta-1; Protein tyrosine phosphatase receptor type f polypeptide-interacting protein-binding protein 1; hSGT2) (FITC); hSGT2; anti-PPFIBP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 1005

• Applicable Applications for anti-PPFIBP1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PPFIBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 587-617 amino acids from the Central region of human PPFIBP1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PPFIBP1 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the PPFIBP1 antibody detected the PPFIBP1 protein (arrow).)

Related Product Information for anti-PPFIBP1 antibody

The protein encoded by this gene is a member of the LAR protein-tyrosine phosphatase-interacting protein (liprin) family. Liprins interact with members of LAR family of transmembrane protein tyrosine phosphatases, which are known to be important for axon guidance and mammary gland development. It has been proposed that liprins are multivalent proteins that form complex structures and act as scaffolds for the recruitment and anchoring of LAR family of tyrosine phosphatases. This protein was found to interact with S100A4, a calcium-binding protein related to tumor invasiveness and metastasis. In vitro experiment demonstrated that the interaction inhibited the phosphorylation of this protein by protein kinase C and protein kinase CK2. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Product Categories/Family for anti-PPFIBP1 antibody

Antibodies; Enzymes; Phosphatase

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Norrmen, C., et al. Blood 115(4):906-909(2010). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007): Olsen, J.V., et al. Cell 127(3):635-648(2006). Jin, J., et al. Curr. Biol. 14(16):1436-1450(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 29294627
• NCBI GeneID: 8496
• NCBI Accession #: NP_003613.2; NP_803193.1
• NCBI GenBank Nucleotide #: NP_003613.2
• UniProt Accession #: Q86W92
• NCBI Official Full Name: liprin-beta-1 isoform 1
• NCBI Official Synonym Full Names: PPFIA binding protein 1
• NCBI Official Symbol: PPFIBP1
• NCBI Official Synonym Symbols: L2; SGT2; hSGT2; hSgt2p
• NCBI Protein Information: liprin-beta-1
• UniProt Protein Name: Liprin-beta-1
• Protein Family: Liprin
• UniProt Gene Name: PPFIBP1
• UniProt Synonym Gene Names: KIAA1230; PTPRF-interacting protein-binding protein 1
• UniProt Entry Name: LIPB1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the LAR protein-tyrosine phosphatase-interacting protein (liprin) family. Liprins interact with members of LAR family of transmembrane protein tyrosine phosphatases, which are known to be important for axon guidance and mammary gland development. It has been proposed that liprins are multivalent proteins that form complex structures and act as scaffolds for the recruitment and anchoring of LAR family of tyrosine phosphatases. This protein was found to interact with S100A4, a calcium-binding protein related to tumor invasiveness and metastasis. In vitro experiment demonstrated that the interaction inhibited the phosphorylation of this protein by protein kinase C and protein kinase CK2. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

liprin beta 1: a member of the LAR protein-tyrosine phosphatase-interacting protein (liprin) family. Liprins interact with members of LAR family of transmembrane protein tyrosine phosphatases, which are known to be important for axon guidance and mammary gland development. It has been proposed that liprins are multivalent proteins that form complex structures and act as scaffolds for the recruitment and anchoring of LAR family of tyrosine phosphatases. This protein was found to interact with S100A4, a calcium-binding protein related to tumor invasiveness and metastasis. In vitro experiment demonstrated that the interaction inhibited the phosphorylation of this protein by protein kinase C and protein kinase CK2. Five alternatively spliced isoforms have been described.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 12p12.1

Cellular Component: focal adhesion; plasma membrane

Biological Process: cell adhesion