Rabbit anti-Human PPEF2 Polyclonal Antibody | anti-PPEF2 antibody

PPEF2, ID (PPEF2, Serine/threonine-protein phosphatase with EF-hands 2) (Azide free) (HRP)

Cat. Num. AAA6336196

• Gene Names: PPEF2; PPP7CB
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PPEF2; Polyclonal Antibody; ID (PPEF2; Serine/threonine-protein phosphatase with EF-hands 2) (Azide free) (HRP); Serine/threonine-protein phosphatase with EF-hands 2; anti-PPEF2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PPEF2 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PPEF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 303-333 amino acids from the Central region of human PPEF2.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PPEF2 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the PPEF2 antibody detected the PPEF2 protein (arrow).)

Immunohistochemistry (IHC)

(PPEF2 Antibody (Center) (AP14402c)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PPEF2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-PPEF2 antibody

This gene encodes a member of the serine/threonine protein phosphatase with EF-hand motif family. The protein contains a protein phosphatase catalytic domain, and at least two EF-hand calcium-binding motifs in its C terminus. Although its substrate(s) is unknown, the encoded protein, which is expressed specifically in photoreceptors and the pineal, has been suggested to play a role in the visual system. This gene shares high sequence similarity with the Drosophila retinal degeneration C (rdgC) gene. [provided by RefSeq].

Product Categories/Family for anti-PPEF2 antibody

Antibodies; Enzymes; Phosphatase

References

Kutuzov, M.A., et al. Biochem. Biophys. Res. Commun. 293(3):1047-1052(2002). Ramulu, P., et al. Mol. Cell. Biol. 21(24):8605-8614(2001). Sherman, P.M., et al. Proc. Natl. Acad. Sci. U.S.A. 94(21):11639-11644(1997).

NCBI and Uniprot Product Information

• NCBI GI #: 23312386
• NCBI GeneID: 5470
• NCBI Accession #: NP_006230.2
• NCBI GenBank Nucleotide #: NM_006239.2
• UniProt Accession #: O14830; O14831
• Molecular Weight: 86,518 Da
• NCBI Official Full Name: serine/threonine-protein phosphatase with EF-hands 2
• NCBI Official Synonym Full Names: protein phosphatase, EF-hand calcium binding domain 2
• NCBI Official Symbol: PPEF2
• NCBI Official Synonym Symbols: PPP7CB
• NCBI Protein Information: serine/threonine-protein phosphatase with EF-hands 2; protein phosphatase with EF hands 2; protein phosphatase, EF hand calcium-binding domain 2; protein phosphatase 7, catalytic subunit, beta isozyme
• UniProt Protein Name: Serine/threonine-protein phosphatase with EF-hands 2
• Protein Family: Serine/threonine-protein phosphatase
• UniProt Gene Name: PPEF2
• UniProt Synonym Gene Names: PPEF-2
• UniProt Entry Name: PPE2_HUMAN