Rabbit anti-Human PPCS Polyclonal Antibody | anti-PPCS antibody

PPCS Polyclonal Antibody

Cat. Num. AAA9132640

• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purification
• Synonyms: PPCS; Polyclonal Antibody; PPCS Polyclonal Antibody; anti-PPCS antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Sequence Length: 311

• Applicable Applications for anti-PPCS antibody: Western Blot (WB)
• Application Notes: WB: 1:500 - 1:2000
• Immunogen: Recombinant protein of human PPCS
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using PPCS antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

Related Product Information for anti-PPCS antibody

Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCS (EC 6.3.2.5), one of the last enzymes in this pathway, converts phosphopantothenate to phosphopantothenoylcysteine (Daugherty et al., 2002 [PubMed 11923312]).

Product Categories/Family for anti-PPCS antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 47117318
• NCBI GeneID: 79717
• UniProt Accession #: Q9HAB8; Q3KQT2; Q5VVM0
• Molecular Weight: 34kDa
• NCBI Official Full Name: Phosphopantothenate--cysteine ligase
• NCBI Official Synonym Full Names: phosphopantothenoylcysteine synthetase
• NCBI Official Symbol: PPCS
• NCBI Protein Information: phosphopantothenate--cysteine ligase
• UniProt Protein Name: Phosphopantothenate--cysteine ligase
• Protein Family: Phosphopantothenate--cysteine ligase
• UniProt Gene Name: PPCS
• UniProt Synonym Gene Names: COAB; PPC synthetase

NCBI Description

Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCS (EC 6.3.2.5), one of the last enzymes in this pathway, converts phosphopantothenate to phosphopantothenoylcysteine (Daugherty et al., 2002 [PubMed 11923312]).[supplied by OMIM, Mar 2008]

Uniprot Description

PPCS: Catalyzes the first step in the biosynthesis of coenzyme A from vitamin B5, where cysteine is conjugated to 4'- phosphopantothenate to form 4-phosphopantothenoylcysteine. Belongs to the PPC synthetase family.

Protein type: Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; EC 6.3.2.5; Ligase

Chromosomal Location of Human Ortholog: 1p34.2

Cellular Component: cytosol

Molecular Function: phosphopantothenate--cysteine ligase activity

Biological Process: coenzyme A biosynthetic process; coenzyme biosynthetic process