Rabbit anti-Human PPARd Polyclonal Antibody | anti-PPARD antibody

PPARd (Peroxisome Proliferator Activated Receptor, delta)

Cat. Num. AAA612577

• Gene Names: PPARD; FAAR; NUC1; NUCI; NR1C2; NUCII; PPARB; MGC3931
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: PPARd; Polyclonal Antibody; PPARd (Peroxisome Proliferator Activated Receptor; delta); Anti -PPARd (Peroxisome Proliferator Activated Receptor; anti-PPARD antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes the human PPAR delta. Expected to react with mouse and rat based on 100% sequence homology. Crossreactivity with PPAR delta from human, chimpanzee and rabbit may occur as this sequence shows 85% homology with the protein from these sources. No reactivity is expected with other subtypesof PPAR.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.

• Applicable Applications for anti-PPARD antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:2000-1:8000; Western Blot: 1:500-1:2000.
• Immunogen: A synthetic peptide, MEQPQEETPEAREE, corresponding to an internal region of mouse PPAR delta.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-PPARD antibody

Since their discovery in the early 1990's, the peroxisome proliferator activated receptors (PPARs) have attracted significant attention. This is primarily because PPARs serve as receptors for two very important classes of drugs: the hypolipidemic fibrates and the insulin sensitizing thiazolidinediones. Peroxisome proliferators are non-genotoxic carcinogens that are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed PPARs. Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Upon binding fatty acids or hypolipidemic drugs, PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate the expression of target genes. There are 3 known subtypes of PPARs: PPAR-alpha, PPAR-delta and PPAR-gamma. Mostly target genes are involved in the catabolism of fatty acids. Conversely, PPAR-gamma is activated by peroxisome proliferators such as prostaglandins, leukotrienes and antidiabetic thiazolidinediones and affects the expression of genes involved in the storage of the fatty acids. PPARgamma may also be involved in adipocyte differentiation. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 through interaction with specific response elements.

Product Categories/Family for anti-PPARD antibody

Antibodies; Abs to Obesity Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 284807159
• NCBI GeneID: 5467
• NCBI Accession #: NP_001165291.1
• NCBI GenBank Nucleotide #: NM_001171820.1
• UniProt Accession #: Q03181; Q5D1P0; Q7Z5K0; Q9BUD4; A8K6J6; B6ZGS1
• Molecular Weight: 49,903 Da
• NCBI Official Full Name: peroxisome proliferator-activated receptor delta isoform 4
• NCBI Official Synonym Full Names: peroxisome proliferator-activated receptor delta
• NCBI Official Symbol: PPARD
• NCBI Official Synonym Symbols: FAAR; NUC1; NUCI; NR1C2; NUCII; PPARB; MGC3931
• NCBI Protein Information: peroxisome proliferator-activated receptor delta; PPAR-beta; PPAR-delta; OTTHUMP00000016256; OTTHUMP00000016257; nuclear hormone receptor 1; nuclear receptor subfamily 1 group C member 2; peroxisome proliferator-activated receptor beta; peroxisome proliferator-activated nuclear receptor beta/delta variant 2
• UniProt Protein Name: Peroxisome proliferator-activated receptor delta
• Protein Family: Peroxisome proliferator-activated receptor
• UniProt Gene Name: PPARD
• UniProt Synonym Gene Names: NR1C2; PPARB
• UniProt Entry Name: PPARD_HUMAN

NCBI Description

This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) family. PPARs are nuclear hormone receptors that bind peroxisome proliferators and control the size and number of peroxisomes produced by cells. PPARs mediate a variety of biological processes, and may be involved in the development of several chronic diseases, including diabetes, obesity, atherosclerosis, and cancer. This protein is a potent inhibitor of ligand-induced transcription activity of PPAR alpha and PPAR gamma. It may function as an integrator of transcription repression and nuclear receptor signaling. The expression of this gene is found to be elevated in colorectal cancer cells. The elevated expression can be repressed by adenomatosis polyposis coli (APC), a tumor suppressor protein related to APC/beta-catenin signaling pathway. Knockout studies in mice suggested the role of this protein in myelination of the corpus callosum, lipid metabolism, and epidermal cell proliferation. Alternate splicing results in multiple transcript variants. [provided by RefSeq]

Uniprot Description

PPAR-delta: Ligand-activated transcription factor. Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand. Belongs to the nuclear hormone receptor family. NR1 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Nuclear receptor

Chromosomal Location of Human Ortholog: 6p21.2

Cellular Component: nucleoplasm; nucleus

Molecular Function: NF-kappaB binding; ligand-dependent nuclear receptor activity; protein binding; DNA binding; zinc ion binding; sequence-specific DNA binding; transcription coactivator activity; steroid hormone receptor activity; drug binding; transcription factor activity; lipid binding

Biological Process: proteoglycan metabolic process; wound healing; negative regulation of smooth muscle cell proliferation; heart development; negative regulation of collagen biosynthetic process; positive regulation of transcription, DNA-dependent; positive regulation of epidermis development; negative regulation of smooth muscle cell migration; glucose transport; negative regulation of transcription from RNA polymerase II promoter; decidualization; positive regulation of vasodilation; vitamin A metabolic process; response to vitamin A; positive regulation of cell proliferation; response to glucose stimulus; axon ensheathment; cell differentiation; negative regulation of epithelial cell proliferation; transcription initiation from RNA polymerase II promoter; cholesterol metabolic process; generation of precursor metabolites and energy; intracellular receptor-mediated signaling pathway; glucose metabolic process; positive regulation of insulin secretion; fatty acid catabolic process; keratinocyte proliferation; keratinocyte migration; cell-substrate adhesion; phospholipid biosynthetic process; regulation of transcription from RNA polymerase II promoter; positive regulation of phosphoinositide 3-kinase cascade; cell proliferation; fatty acid beta-oxidation; negative regulation of inflammatory response; mRNA transcription; positive regulation of fat cell differentiation; steroid hormone mediated signaling; gene expression; response to activity; negative regulation of cell growth; lipid metabolic process; negative regulation of transcription, DNA-dependent; embryo implantation; fatty acid transport; negative regulation of apoptosis