Rabbit PP2A-alpha Polyclonal Antibody | anti-PP2A antibody

PP2A-alpha (T301) Polyclonal Antibody

Cat. Num. AAA3001346

• Gene Names: PPP2CA; RP-C; PP2Ac; PP2CA; NEDLBA; PP2Calpha
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: PP2A-alpha; Polyclonal Antibody; PP2A-alpha (T301) Polyclonal Antibody; Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; Replication protein C; RP-C; PPP2CA; PP2Aalpha; PP2A alpha; anti-PP2A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PP2A-alpha (T301) polyclonal antibody detects endogenous levels of PP2A-alpha protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 309

• Applicable Applications for anti-PP2A antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 270-320 of Human PP2A-alpha.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of PP2A-alpha (T301) polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate (40ug) Lane2:COS-7 whole cell lysate (40ug) Lane3:C6 whole cell lysate (40ug) Lane4:NIH-3T3 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of PP2A-alpha (T301) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-PP2A antibody

In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunits have been identified, designated PP1, PP2A, PP2B (calcineurin) and PP2C. The PP2A family comprises subfamily members PP2Aalpha and PP2Abeta. An additional protein phosphatase catalytic subunit, PPX (also known as PP4) is a putative member of a novel PP family.

NCBI and Uniprot Product Information

• NCBI GI #: 4506017
• NCBI GeneID: 5515
• NCBI Accession #: NP_002706.1
• NCBI GenBank Nucleotide #: NP_002706.1
• UniProt Accession #: P67775
• Molecular Weight: ~ 38kDa
• NCBI Official Full Name: serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform isoform 1
• NCBI Official Synonym Full Names: protein phosphatase 2 catalytic subunit alpha
• NCBI Official Symbol: PPP2CA
• NCBI Official Synonym Symbols: RP-C; PP2Ac; PP2CA; NEDLBA; PP2Calpha
• NCBI Protein Information: serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
• UniProt Protein Name: Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
• UniProt Gene Name: PPP2CA
• UniProt Synonym Gene Names: PP2A-alpha; RP-C
• UniProt Entry Name: PP2AA_HUMAN

NCBI Description

This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq, Jul 2008]

Uniprot Description

PPP2CA: ubiquitous cytosolic Ser/Thr protein phosphatase, catalytic subunit. The holoenzyme consists of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B'/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules.

Protein type: EC 3.1.3.16; Motility/polarity/chemotaxis; Protein phosphatase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: microtubule cytoskeleton; spindle pole; protein phosphatase type 2A complex; membrane; mitochondrion; plasma membrane; nucleus; cytosol; chromosome, pericentric region

Molecular Function: protein dimerization activity; protein C-terminus binding; protein binding; metal ion binding; GABA receptor binding; protein serine/threonine phosphatase activity

Biological Process: mitotic nuclear envelope reassembly; apoptosis; regulation of protein amino acid autophosphorylation; protein amino acid dephosphorylation; response to organic substance; regulation of transcription, DNA-dependent; meiotic cell cycle; regulation of cell differentiation; regulation of growth; mesoderm development; regulation of receptor activity; regulation of DNA replication; inactivation of MAPK activity; second-messenger-mediated signaling; regulation of cell adhesion; fibroblast growth factor receptor signaling pathway; RNA splicing; regulation of Wnt receptor signaling pathway; negative regulation of tyrosine phosphorylation of Stat3 protein; mRNA catabolic process, nonsense-mediated decay; gene expression; regulation of protein catabolic process; mitotic cell cycle; negative regulation of cell growth; ceramide metabolic process