Western Blot (WB)
(Western Blot Analysis: Hela lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-PP-1 GAMMA (1:500 dilution of this lot). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein PP-1 GAMMA (~37kD).)
Rabbit PP1 gamma1 Polyclonal Antibody
PP1 gamma1 (protein phosphatase 1, catalytic subunit, gamma isoform, PP-1G, Protein Phosphatase 1C Catalytic Subunit, Serine/threonine-protein Phosphatase PP1-gamma Catalytic Subunit)
Reactivity
Bovine, Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation
Purity
PurifiedPurified
Synonyms
PP1 gamma1; Polyclonal Antibody; PP1 gamma1 (protein phosphatase 1; catalytic subunit; gamma isoform; PP-1G; Protein Phosphatase 1C Catalytic Subunit; Serine/threonine-protein Phosphatase PP1-gamma Catalytic Subunit); Anti -PP1 gamma1 (protein phosphatase 1; anti-PP1 gamma1 antibody
Host
Rabbit
Reactivity
Bovine, Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes Protein Phosphatase 1 gamma1. No cross reactivity with other recombinant PP1 isoforms. Species Crossreactivity: Human, Mouse, Rat, and Bovine.
Purity/Purification
Purified
Purified
Purified
Form/Format
Supplied as a liquid in sterile filtered PBS, 0.08% sodium azide.
Applicable Applications for anti-PP1 gamma1 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Suitable for use in Western Blot and Immunoprecipitation.
Dilution: Western Blot: 1:500-1:1000
Dilution: Western Blot: 1:500-1:1000
Immunogen
Synthetic peptide corresponding to C -terminus of PP1 gamma 1 catalytic subunit (aa311-323).
Positive Control
HeLa Cell Lysate
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western Blot Analysis: Hela lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-PP-1 GAMMA (1:500 dilution of this lot). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein PP-1 GAMMA (~37kD).)
Western Blot (WB)
(Western Blot Analysis: Hela lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-PP-1 GAMMA (1:500 dilution of this lot). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein PP-1 GAMMA (~37kD).)
Western Blot (WB)
(Western Blot Analysis: Representative image from a previous lot. HEK293 lysate (Lane 1), NIH/3T3 lysate (Lane 2), A431 lysate (Lane 3), L6 lysate (Lane 4), HepG2 lysate (Lane 5) and HeLa lysate (Lane 6) were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-PP-1 GAMMA (1:500 dilution of a previous lot). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein PP-1 GAMMA (~37kD).)
Western Blot (WB)
(Western Blot Analysis: Representative image from a previous lot. HEK293 lysate (Lane 1), NIH/3T3 lysate (Lane 2), A431 lysate (Lane 3), L6 lysate (Lane 4), HepG2 lysate (Lane 5) and HeLa lysate (Lane 6) were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-PP-1 GAMMA (1:500 dilution of a previous lot). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein PP-1 GAMMA (~37kD).)
Related Product Information for anti-PP1 gamma1 antibody
Type 1 protein Ser/Thr phosphatases (PP1) have a high specific activity against phosphorylase, and are resistant to okadaic acid up to concentrations of 50 nM, but sensitive to Inhibitor-2 and microcystin LR.
Product Categories/Family for anti-PP1 gamma1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
protein phosphatase 1 gamma1
Similar Products
Product Notes
The PP1 gamma1 (Catalog #AAA620776) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PP1 gamma1 (protein phosphatase 1, catalytic subunit, gamma isoform, PP-1G, Protein Phosphatase 1C Catalytic Subunit, Serine/threonine-protein Phosphatase PP1-gamma Catalytic Subunit) reacts with Bovine, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PP1 gamma1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). Suitable for use in Western Blot and Immunoprecipitation. Dilution: Western Blot: 1:500-1:1000. Researchers should empirically determine the suitability of the PP1 gamma1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PP1 gamma1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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