Rabbit PP1-alpha Polyclonal Antibody | anti-PP1-alpha antibody

PP1-alpha (Phospho-Thr320) Antibody

Cat. Num. AAA9502627

• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Synonyms: PP1-alpha; Polyclonal Antibody; PP1-alpha (Phospho-Thr320) Antibody; anti-PP1-alpha antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: PP1-alpha (Phospho-Thr320) Antibody detects endogenous levels of PP1-alpha only when phosphorylated at Thr320.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 286

• Applicable Applications for anti-PP1-alpha antibody: Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: IHC: 1:50~1:100; ELISA: 1:10000
• Target Modification: Phospho
• Modification Sites: H:T320; M:T320; R:T320
• Immunogen: The antiserum was produced against synthesized peptide derived from human PP1-alpha around the phosphorylation site of Thr320.
• Immunogen Range: 281-330
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PP1-alpha (Phospho-Thr320) Antibody. The picture on the right is blocked with the phospho peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 56790945
• NCBI GeneID: 5499
• NCBI Accession #: NP_001008709.1
• NCBI GenBank Nucleotide #: NM_001008709.1
• UniProt Accession #: P62136; P08129; P20653; P22802; Q07161; A6NNR3; B2R908
• Molecular Weight: 37 kDa
• NCBI Official Full Name: serine/threonine-protein phosphatase PP1-alpha catalytic subunit isoform 3
• UniProt Protein Name: Serine/threonine-protein phosphatase PP1-alpha catalytic subunit
• UniProt Gene Name: PPP1CA
• UniProt Synonym Gene Names: PPP1A; PP-1A

Uniprot Description

Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Regulates NEK2 function in terms of kinase activity and centrosome number and splitting, both in the presence and absence of radiation-induced DNA damage. Regulator of neural tube and optic fissure closure, and enteric neural crest cell (ENCCs) migration during development. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. Dephosphorylates the 'Ser-418' residue of FOXP3 in regulatory T-cells (Treg) from patients with rheumatoid arthritis, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Dephosphorylates CENPA (PubMed:25556658). Dephosphorylates the 'Ser-139' residue of ATG16L1 causing dissociation of ATG12-ATG5-ATG16L1 complex, thereby inhibiting autophagy (PubMed:26083323).