Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using POU6F1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
Rabbit POU6F1 Polyclonal Antibody | anti-POU6F1 antibody
POU6F1 Polyclonal Antibody
Gene Names
POU6F1; BRN5; MPOU; TCFB1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Synonyms
POU6F1; Polyclonal Antibody; POU6F1 Polyclonal Antibody; BRN5; MPOU; TCFB1; anti-POU6F1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Concentration
1mg/ml (varies by lot)
Sequence Length
301
Applicable Applications for anti-POU6F1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500 - 1:2000
IF: 1:50 - 1:100
IF: 1:50 - 1:100
Species
Human
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human POU6F1
Calculated Molecular Weight
33kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using POU6F1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using POU6F1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
Immunofluorescence (IF)
(Immunofluorescence analysis of MCF-7 cells using POU6F1 antibody.)
Immunofluorescence (IF)
(Immunofluorescence analysis of MCF-7 cells using POU6F1 antibody.)
Product Categories/Family for anti-POU6F1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
301
NCBI Official Full Name
POU domain, class 6, transcription factor 1
NCBI Official Synonym Full Names
POU class 6 homeobox 1
NCBI Official Symbol
POU6F1
NCBI Official Synonym Symbols
BRN5; MPOU; TCFB1
NCBI Protein Information
POU domain, class 6, transcription factor 1; brn-5; brain-5; mPOU homeobox protein; brain-specific homeobox/POU domain protein 5
UniProt Protein Name
POU domain, class 6, transcription factor 1
Protein Family
UniProt Gene Name
POU6F1
UniProt Synonym Gene Names
BRN5; MPOU; TCFB1; Brain-5; Brn-5
UniProt Entry Name
PO6F1_HUMAN
Uniprot Description
POU6F1: Transcription factor that binds preferentially to a variant of the octamer motif (5'-ATGATAAT-3'). Belongs to the POU transcription factor family. Class- 6 subfamily.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 12q13.13
Cellular Component: nucleoplasm; nucleus; actin cytoskeleton
Molecular Function: DNA binding; transcription factor activity
Biological Process: muscle development; transcription, DNA-dependent; regulation of transcription, DNA-dependent; heart development; brain development
Research Articles on POU6F1
1. POU6F1 siRNA dose-dependently suppressed the proliferation of clear cell adenocarcinoma cell lines.
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Product Notes
The POU6F1 pou6f1 (Catalog #AAA9126194) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The POU6F1 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's POU6F1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500 - 1:2000 IF: 1:50 - 1:100. Researchers should empirically determine the suitability of the POU6F1 pou6f1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POU6F1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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