Western Blot (WB)
(Western blot analysis of Oct-3/4 in rat kidney tissue lysate.)
Rabbit POU5F1 Polyclonal Antibody | anti-POU5F1 antibody
POU5F1 Antibody
Gene Names
POU5F1; OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Affinity Chromatography
Synonyms
POU5F1; Polyclonal Antibody; POU5F1 Antibody; Rabbit Polyclonal POU5F1 Antibody; Polyclonal POU5F1 antibody; anti-POU5F1 antibody; OTF4 antibody; POUF1-5; POUF1 5; OTF3 antibody; POUF1 5 antibody; POUF1-5 antibody; Oct-3 antibody; MGC22487 antibody; OCT3 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Chromatography
Form/Format
Supplied in PBS buffer, pH 7.2, containing 30% Glycerol, 0.5% BSA, 0.01% Thimerosal
Concentration
500 ug/ml (varies by lot)
Sequence Length
191
Applicable Applications for anti-POU5F1 antibody
Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
IP: 20 ug/ml
IHC: 20 ug/ml
WB: 0.5-4 ug/ml
IHC: 20 ug/ml
WB: 0.5-4 ug/ml
Immunogen
POU5F1 antibody was raised in Rabbit using synthetic peptide mapping to the N-terminus of mouse Oct-4 as the immunogen.
Cross-Reactivity
Human, Mouse, Rat
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of Oct-3/4 in rat kidney tissue lysate.)
Western Blot (WB)
(Western blot analysis of Oct-3/4 in rat kidney tissue lysate.)
Related Product Information for anti-POU5F1 antibody
Members of Oct family of transcription factors specifically interact with Octamer motif ATGCAAAT, a regulatory element important for tissue- and cell-specific transcription as well as for transcription of a number of housekeeping genes. All of the members of the Oct family contain two highly conserved domains which are separated by 14-26 variable amino acids. These include the POU homeodomain and the POU-specific domain. Both are required for DNA binding and are involved in protein-protein interactions. Evidences indicate that regulation of Oct family transcription factors occurs at the level of phosphorylation.
Product Categories/Family for anti-POU5F1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
POU5F1, partial
NCBI Official Synonym Full Names
POU class 5 homeobox 1
NCBI Official Symbol
POU5F1
NCBI Official Synonym Symbols
OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4
NCBI Protein Information
POU domain, class 5, transcription factor 1
Protein Family
NCBI Description
This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Oct 2013]
Research Articles on POU5F1
Similar Products
Product Notes
The POU5F1 (Catalog #AAA5311372) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's POU5F1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB). IP: 20 ug/ml IHC: 20 ug/ml WB: 0.5-4 ug/ml. Researchers should empirically determine the suitability of the POU5F1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POU5F1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
