Rabbit NFYA Polyclonal Antibody | anti-NFYA antibody

NFYA Antibody

Cat. Num. AAA9504996

• Gene Names: NFYA; HAP2; CBF-A; CBF-B; NF-YA
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using immunogen.
• Synonyms: NFYA; Polyclonal Antibody; NFYA Antibody; anti-NFYA antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: NFYA Antibody detects endogenous levels of total NFYA protein.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 318

• Applicable Applications for anti-NFYA antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; ELISA: 1:20000
• Target Modification: Unmodified/Total
• Immunogen: The antiserum was produced against synthesized peptide derived from human NFYA.
• Immunogen Range: 261-310
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

Western Blot (WB)

(Western blot analysis of lysates from HepG2, 293, and COLO205 cells, using NFYA Antibody. The lane on the right is blocked with the synthesized peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 4505389
• NCBI GeneID: 4800
• NCBI Accession #: NP_002496.1
• NCBI GenBank Nucleotide #: NM_002505.4
• UniProt Accession #: P23511; Q8IXU0
• Molecular Weight: 36 kDa
• NCBI Official Full Name: nuclear transcription factor Y subunit alpha isoform 1
• NCBI Official Synonym Full Names: nuclear transcription factor Y subunit alpha
• NCBI Official Symbol: NFYA
• NCBI Official Synonym Symbols: HAP2; CBF-A; CBF-B; NF-YA
• NCBI Protein Information: nuclear transcription factor Y subunit alpha
• UniProt Protein Name: Nuclear transcription factor Y subunit alpha
• Protein Family: Nuclear transcription factor
• UniProt Gene Name: NFYA
• UniProt Synonym Gene Names: NF-YA

NCBI Description

The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors. NF-YA positively regulates the transcription of the core clock component ARNTL/BMAL1.