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Western blot (WB) (Western blot analysis of extracts from mouse brain, using POU4F1 Ab.)

Rabbit POU4F1 Polyclonal Antibody | anti-POU4F1 antibody

Rabbit POU4F1 Antibody

Gene Names
POU4F1; BRN3A; RDC-1; Oct-T1; brn-3A
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin
Synonyms
POU4F1; Polyclonal Antibody; Rabbit POU4F1 Antibody; anti-POU4F1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
POU4F1 Ab detects endogenous levels of total POU4F1.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin
Form/Format
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-POU4F1 antibody
Western Blot (WB)
Application Notes
WB 1:500-1:1000

IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
Immunogen
A synthesized peptide derived from human POU4F1, corresponding to a region within the internal amino acids.
Subcellular Location
Nucleus.
Tissue Specificity
Expressed in the brain and the retina. Present in the developing brain, spinal cord and eye.
Similarity
The C-terminal domain is able to act as both DNA-binding domain and a transcriptional activator. The N-terminal domain is also required for transactivation activity on some target genes acting as a discrete activation domain. Neurite outgrowth and expression of genes required for synapse formation are primarily dependent on the C-terminal domain, however the N-terminal domain is required for maximal induction.Belongs to the POU transcription factor family. Class-4 subfamily.
Preparation and Storage
Store at -20°C. Stable for 12 months from date of receipt.

Western blot (WB)

(Western blot analysis of extracts from mouse brain, using POU4F1 Ab.)

Western blot (WB) (Western blot analysis of extracts from mouse brain, using POU4F1 Ab.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
42 KD
NCBI Official Full Name
POU domain, class 4, transcription factor 1
NCBI Official Synonym Full Names
POU class 4 homeobox 1
NCBI Official Symbol
POU4F1
NCBI Official Synonym Symbols
BRN3A; RDC-1; Oct-T1; brn-3A
NCBI Protein Information
POU domain, class 4, transcription factor 1; brain-3A; homeobox/POU domain protein RDC-1; brain-specific homeobox/POU domain protein 3A
UniProt Protein Name
POU domain, class 4, transcription factor 1
UniProt Gene Name
POU4F1
UniProt Synonym Gene Names
BRN3A; RDC1; Brain-3A; Brn-3A
UniProt Entry Name
PO4F1_HUMAN

NCBI Description

This gene encodes a member of the POU-IV class of neural transcription factors. This protein is expressed in a subset of retinal ganglion cells and may be involved in the developing sensory nervous system. This protein may also promote the growth of cervical tumors. A translocation of this gene is associated with some adult acute myeloid leukemias. [provided by RefSeq, Mar 2012]

Uniprot Description

POU4F1: Probable transcription factor which may play a role in the regulation of specific gene expression within a subset of neuronal lineages. May play a role in determining or maintaining the identities of a small subset of visual system neurons. Belongs to the POU transcription factor family. Class- 4 subfamily.

Protein type: DNA-binding; Transcription factor; Cell development/differentiation

Chromosomal Location of Human Ortholog: 13q31.1

Cellular Component: neuron projection; nuclear chromatin

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding

Biological Process: transcription from RNA polymerase II promoter; central nervous system neuron differentiation; trigeminal nerve development; positive regulation of apoptosis; negative regulation of transcription from RNA polymerase II promoter; habenula development; peripheral nervous system neuron development; cell migration in hindbrain; proprioception during equilibrioception; regulation of neurogenesis; synaptogenesis; suckling behavior; axonogenesis; neuron fate specification; mesoderm development; positive regulation of transcription from RNA polymerase II promoter; sensory system development; negative regulation of neuron apoptosis

Research Articles on POU4F1

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Product Notes

The POU4F1 pou4f1 (Catalog #AAA000749) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit POU4F1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's POU4F1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB 1:500-1:1000 IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight. Researchers should empirically determine the suitability of the POU4F1 pou4f1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POU4F1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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