Human Glycerol-3-Phosphate Acyltransferase, Mitochondrial (GPAM) ELISA Kit | GPAM elisa kit

Human Glycerol-3-Phosphate Acyltransferase, Mitochondrial (GPAM) ELISA Kit

Cat. Num. AAA451140

• Gene Names: GPAM; GPAT; GPAT1
• Reactivity: Human
• Synonyms: Glycerol-3-Phosphate Acyltransferase; Mitochondrial (GPAM); Human Glycerol-3-Phosphate Acyltransferase; Mitochondrial (GPAM) ELISA Kit; GPAT1; GPAM elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of GPAM. No significant cross-reactivity or interference between GPAM and analogues was observed.
• Sequence Length: 828

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.059ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GPAM were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GPAM were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Standard Curve/Testing Data

Related Product Information for GPAM elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GPAM in human tissue homogenates, cell lysates, and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GPAM. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to GPAM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GPAM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GPAM in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 350276140
• NCBI GeneID: 57678
• NCBI Accession #: NP_001231878.1
• NCBI GenBank Nucleotide #: NM_001244949.1
• UniProt Accession #: Q9HCL2; Q5VW51; Q86TA3
• Molecular Weight: 93,795 Da
• NCBI Official Full Name: glycerol-3-phosphate acyltransferase 1, mitochondrial
• NCBI Official Synonym Full Names: glycerol-3-phosphate acyltransferase, mitochondrial
• NCBI Official Symbol: GPAM
• NCBI Official Synonym Symbols: GPAT; GPAT1
• NCBI Protein Information: glycerol-3-phosphate acyltransferase 1, mitochondrial
• UniProt Protein Name: Glycerol-3-phosphate acyltransferase 1, mitochondrial
• UniProt Gene Name: GPAM
• UniProt Synonym Gene Names: GPAT1; KIAA1560; GPAT-1

NCBI Description

This gene encodes a mitochondrial enzyme which prefers saturated fatty acids as its substrate for the synthesis of glycerolipids. This metabolic pathway's first step is catalyzed by the encoded enzyme. Two forms for this enzyme exist, one in the mitochondria and one in the endoplasmic reticulum. Two alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis.