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Testing Data (Fig-1: Expression analysis of POU3F2. Anti-POU3F2 antibody (11-12016) was used at 1 ug/ml on (1) K562, (2) HCT-116 and (3) 293 lysates)

Rabbit POU3F2 Polyclonal Antibody | anti-POU3F2 antibody

Polyclonal Antibody to POU3F2

Gene Names
POU3F2; BRN2; OCT7; OTF7; OTF-7; POUF3; brn-2; oct-7; N-Oct3
Reactivity
Human
Predicted: Mouse
Applications
Western Blot
Purity
Purified
Protein A Chromatography
Synonyms
POU3F2; Polyclonal Antibody; Polyclonal Antibody to POU3F2; BRN2; OCT7; OTF7; Brain-specific homeobox/POU domain protein 2; Nervous system-specific octamer-binding transcription factor N-Oct-3; Oct-7; Octamer-binding protein 7; OTF-7; Octamer-binding transcription factor 7; Antibody to POU3F2; Anti-POU3F2 Antibody; POU3F2 Antibody; anti-POU3F2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Predicted: Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified
Protein A Chromatography
Form/Format
25 ug in 50 ul/100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Sequence Length
443
Applicable Applications for anti-POU3F2 antibody
Western Blot (WB)
Application Notes
WB: 1-2 ug/ml
Immunogen Information
A partial length recombinant POU3F2 protein (amino acids 220-421) was used as the immunogen for this antibody.
Antigen
POU3F2
Preparation and Storage
Store the antibody at 4 degree C, stable for 6 months.
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.

Testing Data

(Fig-1: Expression analysis of POU3F2. Anti-POU3F2 antibody (11-12016) was used at 1 ug/ml on (1) K562, (2) HCT-116 and (3) 293 lysates)

Testing Data (Fig-1: Expression analysis of POU3F2. Anti-POU3F2 antibody (11-12016) was used at 1 ug/ml on (1) K562, (2) HCT-116 and (3) 293 lysates)
Related Product Information for anti-POU3F2 antibody
POU3F2 is a developmental neural cell-specific POU domain transcription factor and is crucial for cell lineage determination. In melanoma lesions, cells expressing high levels of POU3F2 show enhanced invasive and metastatic capacity that can in part be explained by repression of MITF (Micropthalmia-associated Transcription Factor) expression via POU3F2 binding to its promoter. POU3F2 is expressed exclusively in the central nervous system, more specifically in postmitotic neurons and glia.
Product Categories/Family for anti-POU3F2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
26,841 Da
NCBI Official Full Name
POU domain, class 3, transcription factor 2
NCBI Official Synonym Full Names
POU class 3 homeobox 2
NCBI Official Symbol
POU3F2
NCBI Official Synonym Symbols
BRN2; OCT7; OTF7; OTF-7; POUF3; brn-2; oct-7; N-Oct3
NCBI Protein Information
POU domain, class 3, transcription factor 2
UniProt Protein Name
POU domain, class 3, transcription factor 2
UniProt Gene Name
POU3F2
UniProt Synonym Gene Names
BRN2; OCT7; OTF7; Brain-2; Brn-2; Oct-7; OTF-7

NCBI Description

This gene encodes a member of the POU-III class of neural transcription factors. The encoded protein is involved in neuronal differentiation and enhances the activation of corticotropin-releasing hormone regulated genes. Overexpression of this protein is associated with an increase in the proliferation of melanoma cells. [provided by RefSeq, Mar 2012]

Uniprot Description

Oct7: Transcription factor that binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a nonconserved spacer region of 0, 2, or 3 nucleotides. Positively regulates the genes under the control of corticotropin-releasing hormone (CRH) and CRH II promoters. Interacts with PQBP1. Expressed specifically in the neuroectodermal cell lineage. Belongs to the POU transcription factor family. Class- 3 subfamily. 3 isoforms of the human protein are produced by alternative initiation.

Protein type: DNA-binding; Motility/polarity/chemotaxis; Transcription factor

Chromosomal Location of Human Ortholog: 6q16.1

Molecular Function: identical protein binding; protein binding; transcription factor activity

Biological Process: positive regulation of cell proliferation

Research Articles on POU3F2

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Product Notes

The POU3F2 pou3f2 (Catalog #AAA668756) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to POU3F2 reacts with Human Predicted: Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's POU3F2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1-2 ug/ml. Researchers should empirically determine the suitability of the POU3F2 pou3f2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POU3F2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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