Rabbit POU2AF1 Polyclonal Antibody | anti-POU2AF1 antibody

POU2AF1 Polyclonal Antibody

Cat. Num. AAA3011677

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: POU2AF1; Polyclonal Antibody; POU2AF1 Polyclonal Antibody; POU domain class 2-associating factor 1; B-cell-specific coactivator OBF-1; BOB-1; OCA-B; OCT-binding factor 1; OBF1; anti-POU2AF1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: POU2AF1 polyclonal antibody detects endogenous levels of POU2AF1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 256

• Applicable Applications for anti-POU2AF1 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IF: 1:50-1:200
• Immunogen: Recombinant full length Human POU2AF1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of POU2AF1 polyclonal antibody)

Related Product Information for anti-POU2AF1 antibody

POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. The POU dimer from the OCT1 gene formed on the palindromic OCT factor recognition element, or PORE (ATTTGAAATGCAAAT), could recruit the transcriptional coactivator OBF1. Studies of tissue-specific expression of immunoglobulin promoters demonstrate the importance of an octamer, ATTTGCAT, and the proteins that bind to it. This is a regulatory element important for tissue- and cell-specific transcription as well as for transcription of a number of housekeeping genes. Oct-1 encodes one protein, NF-A1, which is found in nuclear extracts from all cell types and thus is not specific to lymphoid cells as is the protein NF-A2, which is encoded by Oct-2. A novel protein designated Bob 1 (B cell Oct binding protein 1), alternatively called OBF-1, specifically interacts with Oct-1 and Oct-2, enhancing their transcriptional efficacy. Bob 1 is expressed at highest levels in spleen and peripheral blood leukocytes and represents an Oct co-factor capable of conferring cell-specific activation of Oct-1 and Oct-2. Although having no intrinsic capacity for DNA binding, Bob 1 associates tightly with the octamer motif in the presence of Oct-1 and/or Oct-2. The gene which encodes Bob 1 maps to human chromosome 11q23.1.

NCBI and Uniprot Product Information

• NCBI GI #: 167900478
• NCBI Accession #: NP_006226.2
• NCBI GenBank Nucleotide #: NP_006226.2
• UniProt Accession #: Q16633
• Molecular Weight: ~ 27kDa
• NCBI Official Full Name: POU domain class 2-associating factor 1
• UniProt Protein Name: POU domain class 2-associating factor 1
• Protein Family: POU domain class 2-associating factor
• UniProt Gene Name: POU2AF1
• UniProt Synonym Gene Names: OBF1
• UniProt Entry Name: OBF1_HUMAN

Uniprot Description

POU2AF1: Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6. Belongs to the POU2AF1 family.

Protein type: Transcription, coactivator/corepressor; Oncoprotein

Chromosomal Location of Human Ortholog: 11q23.1

Cellular Component: nucleus

Molecular Function: protein binding; DNA binding; transcription coactivator activity; transcription cofactor activity

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; humoral immune response