Rat anti-Mouse CD162 Monoclonal Antibody | anti-CD162 antibody

Anti-Mouse CD162 Monoclonal Antibody (PerCP/Cyanine5.5 Conjugated) [4RA10]

Cat. Num. AAA2558273

• Gene Names: Selplg; CD162; Psgl1; Selp1; Selpl; Psgl-1
• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD162; Monoclonal Antibody; Anti-Mouse CD162 Monoclonal Antibody (PerCP/Cyanine5.5 Conjugated) [4RA10]; P-selectin glycoprotein ligand 1; Selplg; PSGL-1; Selectin P ligand; anti-CD162 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: 4RA10
• Form/Format: PBS with 0.05% Proclin300, 1% BSA
• Concentration: 0.5 mg/mL (varies by lot)
• Sequence Length: 397

• Applicable Applications for anti-CD162 antibody: Flow Cytometry (FC/FACS)
• Application Notes: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is <= 4 ug per 10⁶ cells in 100 ul volume or 100 ul of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
• Conjugation: PErCP/Cyanine5.5
• Preparation and Storage: Store at 2-8 degree C and protect from prolonged exposure to light. Do not freeze.

Flow Cytometry (FC/FACS)

(C57BL/6 murine splenocytes are stained with Anti-Mouse CD162 Monoclonal Antibody(PerCP/Cyanine5.5 Conjugated)(filled gray histogram). Unstained splenocytes (empty black histogram) are used as control.)

Related Product Information for anti-CD162 antibody

The 4RA10 antibody reacts with the N-terminal functional peptide of CD162 (P-selectin glycoprotein ligand-1, PSGL-1), encoded by the Selpl gene. PSGL-1 is expressed on the cell surface as a homodimer of approximately 230 kDa. In the mouse, Selpl mRNA is detected in most tissues, with high levels found in hematopoietic cells, brain, and adipose tissue. Flow cytometric analyses have revealed CD162 expression on bone marrow-derived mast and dendritic cells, splenic leukocytes, platelets, peripheral blood neutrophils, and neutrophil and T-cell lines. PSGL-1 is a ligand for P-selectin (CD62P) and is involved in leukocyte rolling, the migration of leukocytes into inflamed tissues, and responses to vascular injury. It is a sialomucin that must be specifically sialylated, fucosylated, and sulfated to bind P-selectin. There is also evidence that other ligands for PSGL-1 and CD62P may exist. 4RA10 mAb is reported to block the binding of mouse leukocytes to CD62P and CD62L.

NCBI and Uniprot Product Information

• NCBI GI #: 341942014
• NCBI GeneID: 20345
• UniProt Accession #: Q62170
• NCBI Official Full Name: P-selectin glycoprotein ligand 1
• NCBI Official Synonym Full Names: selectin, platelet (p-selectin) ligand
• NCBI Official Symbol: Selplg
• NCBI Official Synonym Symbols: CD162; Psgl1; Selp1; Selpl; Psgl-1
• NCBI Protein Information: P-selectin glycoprotein ligand 1
• UniProt Protein Name: P-selectin glycoprotein ligand 1
• UniProt Gene Name: Selplg
• UniProt Synonym Gene Names: Selp1; Selpl; PSGL-1

Uniprot Description

A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E- and P-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.