Rabbit anti-Human POLR3F Polyclonal Antibody | anti-POLR3F antibody

POLR3F (DNA-directed RNA Polymerase III Subunit RPC6, RNA Polymerase III Subunit C6, DNA-directed RNA Polymerase III Subunit F, RNA Polymerase III 39kD Subunit, POLR3F) APC

Cat. Num. AAA6390176

• Gene Names: POLR3F; RPC6; RPC39
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: POLR3F; Polyclonal Antibody; POLR3F (DNA-directed RNA Polymerase III Subunit RPC6; RNA Polymerase III Subunit C6; DNA-directed RNA Polymerase III Subunit F; RNA Polymerase III 39kD Subunit; POLR3F) APC; anti-POLR3F antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human POLR3F.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-POLR3F antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full-length human POLR3F, aa1-316 (NP_006457.2).
• Immunogen Sequence: MAEVKVKVQPPDADPVEIENRIIELCHQFPHGITDQVIQNEMPHIEAQQRAVAINRLLSMGQLDLLRSNTGLLYRIKDSQNAGKMKGSDNQEKLVYQIIEDAGNKGIWSRDIRYKSNLPLTEINKILKNLESKKLIKAVKSVAASKKKVYMLYNLQPDRSVTGGAWYSDQDFESEFVEVLNQQCFKFLQSKAETARESKQNPMIQRNSSFASSHEVWKYICELGISKVELSMEDIETILNTLIYDGKVEMTIIAAKEGTVGSVDGHMKLYRAVNPIIPPTGLVRAPCGLCPVFDDCHEGGEISPSNCIYMTEWLEF
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(POLR3F rabbit polyclonal antibody. Western Blot analysis of POLR3F expression in human liver.)

Western Blot (WB)

(POLR3F rabbit polyclonal antibody. Western Blot analysis of POLR3F expression in HeLa.)

Western Blot (WB)

(Western Blot analysis of POLR3F expression in transfected 293T cell line by POLR3F polyclonal antibody. Lane 1: POLR3F transfected lysate (35.7kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-POLR3F antibody

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct RNA Pol III binding to the TFIIIB-DNA complex. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF-Kappa-B through the RIG-I pathway.

Product Categories/Family for anti-POLR3F antibody

Antibodies; Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 33598952
• NCBI GeneID: 10621
• NCBI Accession #: NP_006457.2
• NCBI GenBank Nucleotide #: NM_006466.2
• UniProt Accession #: Q9H1D9; O15319; A8K4C7
• Molecular Weight: 35,684 Da
• NCBI Official Full Name: DNA-directed RNA polymerase III subunit RPC6 isoform 1
• NCBI Official Synonym Full Names: polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa
• NCBI Official Symbol: POLR3F
• NCBI Official Synonym Symbols: RPC6; RPC39
• NCBI Protein Information: DNA-directed RNA polymerase III subunit RPC6; RNA polymerase III subunit C6; RNA polymerase III C39 subunit
• UniProt Protein Name: DNA-directed RNA polymerase III subunit RPC6
• UniProt Gene Name: POLR3F
• UniProt Synonym Gene Names: RNA polymerase III subunit C6; RPC39
• UniProt Entry Name: RPC6_HUMAN

NCBI Description

The protein encoded by this gene is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB). Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Uniprot Description

POLR3F: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct RNA Pol III binding to the TFIIIB-DNA complex. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. Belongs to the eukaryotic RPC34/RPC39 RNA polymerase subunit family.

Protein type: Transcription initiation complex; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine

Chromosomal Location of Human Ortholog: 20p11.23

Cellular Component: nucleoplasm; DNA-directed RNA polymerase III complex; cytosol

Molecular Function: protein binding; DNA binding; DNA-directed RNA polymerase activity

Biological Process: transcription from RNA polymerase III promoter; termination of RNA polymerase III transcription; regulation of transcription from RNA polymerase III promoter; positive regulation of innate immune response; positive regulation of interferon type I production; positive regulation of interferon-beta production; innate immune response; gene expression; defense response to virus; RNA elongation from RNA polymerase III promoter