Rabbit anti-Human POLR3D Polyclonal Antibody | anti-POLR3D antibody

POLR3D, ID (POLR3D, BN51, BN51T, DNA-directed RNA polymerase III subunit RPC4, DNA-directed RNA polymerase III subunit D, Protein BN51, RNA polymerase III 47kD subunit, RPC53 homolog) (AP)

Cat. Num. AAA6335796

• Gene Names: POLR3D; RPC4; BN51T; RPC53; TSBN51
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: POLR3D; Polyclonal Antibody; ID (POLR3D; BN51; BN51T; DNA-directed RNA polymerase III subunit RPC4; DNA-directed RNA polymerase III subunit D; Protein BN51; RNA polymerase III 47kD subunit; RPC53 homolog) (AP); RPC53 homolog; anti-POLR3D antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-POLR3D antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: POLR3D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 126-156 amino acids from the Central region of human POLR3D.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(POLR3D Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the POLR3D antibody detected the POLR3D protein (arrow).)

Related Product Information for anti-POLR3D antibody

This gene complements a temperature-sensitive mutant isolated from the BHK-21 Syrian hamster cell line. It leads to a block in progression through the G1 phase of the cell cycle at nonpermissive temperatures.

Product Categories/Family for anti-POLR3D antibody

Antibodies; Enzymes; Polymerase

References

Chiu, Y.H., et al. Cell 138(3):576-591(2009). Lamesch, P., et al. Genomics 89(3):307-315(2007). Olsen, J.V., et al. Cell 127(3):635-648(2006). Olsen, J.V., et al. Cell 127(3):635-648(2006). Hu, P., et al. Mol. Cell. Biol. 22(22):8044-8055(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 55769552
• NCBI GeneID: 661
• NCBI Accession #: NP_001713.2
• NCBI GenBank Nucleotide #: NM_001722.2
• UniProt Accession #: P05423; Q6FI28; Q9BPV7; Q9BPZ1; Q9BXB3
• Molecular Weight: 44,396 Da
• NCBI Official Full Name: DNA-directed RNA polymerase III subunit RPC4
• NCBI Official Synonym Full Names: polymerase (RNA) III (DNA directed) polypeptide D, 44kDa
• NCBI Official Symbol: POLR3D
• NCBI Official Synonym Symbols: RPC4; BN51T; RPC53; TSBN51
• NCBI Protein Information: DNA-directed RNA polymerase III subunit RPC4; RPC53 homolog; RNA polymerase III subunit C4; RNA polymerase III 47 kDa subunit; DNA-directed RNA polymerase III subunit D; BN51 (BHK21) temperature sensitivity complementing; DNA-directed RNA polymerase III 4
• UniProt Protein Name: DNA-directed RNA polymerase III subunit RPC4
• UniProt Gene Name: POLR3D
• UniProt Synonym Gene Names: BN51; BN51T; RNA polymerase III subunit C4
• UniProt Entry Name: RPC4_HUMAN

NCBI Description

This gene complements a temperature-sensitive mutant isolated from the BHK-21 Syrian hamster cell line. It leads to a block in progression through the G1 phase of the cell cycle at nonpermissive temperatures. [provided by RefSeq, Jul 2008]

Uniprot Description

POLR3D: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. Belongs to the eukaryotic RPC4/POLR3D RNA polymerase subunit family.

Protein type: Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 8q21

Cellular Component: nucleoplasm; DNA-directed RNA polymerase III complex; cytoplasm; nuclear chromatin; cytosol

Molecular Function: DNA binding; chromatin binding

Biological Process: transcription from RNA polymerase III promoter; termination of RNA polymerase III transcription; positive regulation of innate immune response; positive regulation of interferon-beta production; positive regulation of interferon type I production; innate immune response; gene expression; defense response to virus; RNA elongation from RNA polymerase III promoter