Rabbit POLR3A Polyclonal Antibody | anti-POLR3A antibody

POLR3A (P68) Polyclonal Antibody

Cat. Num. AAA3003601

• Gene Names: POLR3A; ADDH; C160; HLD7; RPC1; WDRTS; RPC155; hRPC155
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: POLR3A; Polyclonal Antibody; POLR3A (P68) Polyclonal Antibody; DNA-directed RNA polymerase III subunit RPC1; RNA polymerase III subunit C1; EC=2.7.7.6; DNA-directed RNA polymerase III largest subunit; DNA-directed RNA polymerase III subunit A; RNA polymerase III 155 kDa subunit; Short name=RPC155; RNA polymerase III subunit C160; anti-POLR3A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: POLR3A (P68) polyclonal antibody detects endogenous levels of POLR3A protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1390

• Applicable Applications for anti-POLR3A antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 41-90 of Human POLR3A.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of POLR3A (P68) pAb at 1:500 dilutionLane1:PC3 whole cell lysate (40ug) Lane2:Hela whole cell lysate (40ug) Lane3:K562 whole cell lysate (40ug) Lane4:C6 whole cell lysate (40ug) Lane5:BV2 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of POLR3A (P68) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-POLR3A antibody

POLR3A (polymerase (RNA) III (DNA directed) polypeptide A), also known as RPC1 or RPC155, is a 1, 390 amino acid protein that localizes to the nucleus and belongs to the RNA polymerase beta chain family. Existing as the largest and catalytic core component of Pol III, POLR3A functions as a DNA-dependent RNA polymerase that catalyzes the conversion of a nucleoside triphosphate into a diphosphate, thereby transcribing DNA into RNA. The gene encoding POLR3A maps to human chromosome 10, which houses over 1, 200 genes and comprises nearly 4.5% of the human genome.

NCBI and Uniprot Product Information

• NCBI GI #: 39725938
• NCBI GeneID: 11128
• NCBI Accession #: NP_008986.2
• NCBI GenBank Nucleotide #: NP_008986.2
• UniProt Accession #: O14802
• Molecular Weight: ~ 156kDa
• NCBI Official Full Name: DNA-directed RNA polymerase III subunit RPC1
• NCBI Official Synonym Full Names: RNA polymerase III subunit A
• NCBI Official Symbol: POLR3A
• NCBI Official Synonym Symbols: ADDH; C160; HLD7; RPC1; WDRTS; RPC155; hRPC155
• NCBI Protein Information: DNA-directed RNA polymerase III subunit RPC1
• UniProt Protein Name: DNA-directed RNA polymerase III subunit RPC1
• UniProt Gene Name: POLR3A
• UniProt Synonym Gene Names: RNA polymerase III subunit C1; RPC155
• UniProt Entry Name: RPC1_HUMAN

NCBI Description

The protein encoded by this gene is the catalytic component of RNA polymerase III, which synthesizes small RNAs. The encoded protein also acts as a sensor to detect foreign DNA and trigger an innate immune response. [provided by RefSeq, Aug 2011]

Uniprot Description

POLR3A: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. Defects in POLR3A are the cause of leukodystrophy hypomyelinating type 7 with or without oligodontia and/or hypogonadotropic hypogonadism (HLD7). An autosomal recessive neurodegenerative disorder characterized by childhood onset of progressive motor decline manifest as spasticity, ataxia, tremor, and cerebellar signs, as well as mild cognitive regression. Other features may include hypodontia or oligodontia and hypogonadotropic hypogonadism. There is considerable inter- and intrafamilial variability. Belongs to the RNA polymerase beta' chain family.

Protein type: Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine; Transcription initiation complex; Transferase; EC 2.7.7.6

Chromosomal Location of Human Ortholog: 10q22.3

Cellular Component: nucleoplasm; DNA-directed RNA polymerase III complex; membrane; cytosol

Molecular Function: DNA binding; zinc ion binding; ribonucleoside binding; DNA-directed RNA polymerase activity; chromatin binding

Biological Process: transcription from RNA polymerase III promoter; termination of RNA polymerase III transcription; transcription, DNA-dependent; positive regulation of interferon type I production; positive regulation of interferon-beta production; innate immune response; gene expression; defense response to virus; RNA elongation from RNA polymerase III promoter

Disease: Leukodystrophy, Hypomyelinating, 7, With Or Without Oligodontia And/or Hypogonadotropic Hypogonadism